How does the mild nature of the biological buffer BIS-TRIS 6976-37-0 enhance cell viability?

In the fields of biology and biochemistry, the stability of the internal environment of cells is crucial for maintaining normal physiological functions, with precise regulation of pH and ion concentration being particularly important. Bis (2-hydroxyethyl) amino (trihydroxymethyl) methane, also known as BIS-TRIS, plays an important role in enhancing cell viability as a high-quality biological buffer due to its unique chemical properties and mild buffering properties.

1, Basic characteristics of BIS-TRIS

BIS-TRIS, The chemical formula is C8H19NO5, with a molecular weight of 209.24 and a CAS number of 6976-37-0. It is a white powdery crystal that is easily soluble in water. When stored, it should be sealed and placed in a cool, dry place. The buffering range of BIS-TRIS covers pH 5.8-7.2, making it an ideal choice for biochemical experiments. Its structure is similar to Tris reagent, but it has better temperature stability, multiple hydroxyl groups in the molecule, strong hydrophilicity, and no interference with biomolecules (such as enzymes and proteins), making it suitable for long-term experiments.

BIS-TRIS powder

2, Gentle buffering properties of BIS-TRIS

The mild characteristics of BIS-TRIS are mainly reflected in the following aspects:

Stable pH regulation ability: The buffering range of BIS-TRIS covers the pH range required for cell growth and metabolism, effectively maintaining pH stability in the cell culture environment, which is crucial for cell growth, division, and metabolic activity.

Good biocompatibility: BIS-TRIS has no toxic effect on cells and no interference with biomolecules, which means that during cell culture, BIS-TRIS will not interfere with the normal physiological functions of cells, thereby ensuring the enhancement of cell activity.

Temperature stability: BIS-TRIS can remain stable at common room temperatures, which means that under different experimental conditions, BIS-TRIS can maintain its buffering capacity and provide a stable growth environment for cells.

Low ionic strength: BIS-TRIS has a lower ionic strength, which is beneficial for the stability and activity of proteins. In cell culture, a low ion strength environment can reduce ion interference with the cell membrane, thereby protecting the integrity and stability of the cells.

How BIS-TRIS enhances cell viability

The mild characteristics of BIS-TRIS in enhancing cell activity are mainly reflected in the following aspects:

Maintaining pH stability: BIS-TRIS provides a suitable pH environment for cells through its stable pH regulation ability, promoting cell growth and division, while maintaining enzyme activity within the cell and ensuring normal metabolic activity.

Protecting cells from ion interference: BIS-TRIS can absorb or release protons to maintain ion balance in solution, reduce ion interference on cells, and protect cells from damage caused by fluctuations in ion concentration.

Promoting protein stability and activity: BIS-TRIS helps maintain protein stability and activity by maintaining a stable pH and low ionic strength environment, thereby enhancing cellular physiological functions.

Supporting long-term experiments: BIS-TRIS has no interference with biomolecules and is easy to prepare and purify, making it an ideal choice for long-term experiments. In long-term cell culture experiments, BIS-TRIS can continuously provide a stable growth environment for cells, ensuring the accuracy and reliability of experimental results.

Product packaging

As a professional manufacturer and supplier of biological buffer agents, Desheng is committed to providing reliable BIS-TRIS and other biological buffer products for researchers. Our production process is stable, with small batch differences, high product purity, good water solubility, and stable chemical properties. If you are interested, please click on the official website to learn more details or contact me!