The first choice for low-temperature experiments: exploring the excellent performance of BICINE buffer

Low temperature experiments play a crucial role in scientific research and industrial applications. Whether in biochemical research, drug development, or material science exploration, low-temperature environments often reveal properties and reactions of substances that are difficult to observe under conventional conditions. However, experiments under low temperature conditions have raised higher requirements for the stability, solubility, and pH adjustment ability of buffering agents. Among numerous buffering agents, N, N-dihydroxyethylglycine (BICINE buffer) has become one of the preferred choices for low-temperature experiments due to its unique properties.

Basic characteristics of BICINE buffer

N. N-dihydroxyethylglycine, also known as BICINE, is a zwitterionic buffering agent with two hydroxyethyl groups in its molecular structure, endowing it with excellent water solubility and biocompatibility. BICINE has a wide effective pH range, typically between 6.8 and 8.2, which enables it to provide a stable pH environment in various biochemical reactions. It is particularly important that BICINE maintains good buffering capacity and solubility at low temperatures, which is crucial for experiments that require precise pH control and large temperature changes.

bicine powder

Stable performance at low temperatures

In low-temperature experiments, the solubility of many common buffering agents can significantly decrease, and even precipitate crystals, which not only affects the accuracy of experimental results but may also cause damage to experimental materials. In contrast, BICINE buffer can still maintain high solubility under low temperature conditions, ensuring the uniformity and stability of the experimental solution. In addition, BICINE's buffer capacity is almost unaffected at low temperatures, effectively resisting pH fluctuations caused by temperature changes and providing a reliable pH environment for experiments.

Biocompatibility and Wide Application

BICINE buffer is highly favored in biomedical research due to its excellent biocompatibility. It does not interfere with cellular metabolism and does not cause significant cytotoxicity, making BICINE an ideal choice in fields such as cell culture, enzyme reactions, and protein stability research. Especially when storing and transporting biological samples at low temperatures, BICINE can maintain the physiological state of the samples and reduce protein denaturation or enzyme activity loss caused by pH changes.

Practical application cases

1. Protein crystallization research: In protein crystallization experiments, low temperature helps slow down the crystallization rate and improve crystal quality. BICINE, as a buffering agent, not only provides a stable pH environment, but also promotes the ordered arrangement of proteins and optimizes the crystallization process through its unique chemical structure.

2. Enzyme activity assay: Some enzymes exhibit different catalytic activities at low temperatures. Using BICINE buffer for enzyme activity assay can accurately reflect the functional characteristics of enzymes at different temperatures, providing strong support for drug screening and enzyme engineering.

3. Gene expression analysis: BICINE, as a buffer, can effectively protect RNA from degradation and improve the accuracy and sensitivity of gene expression analysis during RNA extraction and reverse transcription under low temperature conditions.

Product packaging

Bicine and other biological buffering agents produced by Hubei Xindesheng Material Technology Co., Ltd. have high purity and good water solubility. Each batch of goods has relevant quality analysis reports to ensure product quality. Desheng has a professional technical team and quality inspectors who can provide excellent technical support and after-sales service. If you have any purchasing needs in the near future, please feel free to contact me or check the official website of Desheng!