EPPS buffer 16052-06-5: the "golden partner" for efficient separation of DNA RNA

In today's rapidly changing field of molecular biology and biotechnology, efficient and pure separation of DNA and RNA is the cornerstone of gene research, disease diagnosis, and biopharmaceuticals. The success of this process largely depends on the performance of the buffer used. Among numerous buffering agents, N - (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid (EPPS buffer) has become the "golden partner" for efficient separation of DNA/RNA due to its unique physicochemical properties and biocompatibility.

EPPS: The unique charm of chemical structure

EPPS, As a zwitterionic buffering agent, its molecular structure cleverly combines the characteristics of positive and negative charge balance, enabling it to maintain stable buffering capacity over a wide pH range. This characteristic is crucial for maintaining the structural integrity of biomolecules, especially nucleic acids, during the extraction process.

EPPS powder

Efficient Separation: Outstanding Performance of EPPS

1. The effective buffering pH range of EPPS is wide, covering most ideal pH values required for DNA/RNA extraction. This wide applicability makes EPPS the preferred buffer for processing various biological samples such as blood, tissues, cell cultures, etc., ensuring high-quality nucleic acid products can be obtained under different experimental conditions.

2. Low ionic strength and low toxicity are another major advantage of EPPS, which helps reduce the interaction between nucleic acids and impurities (such as proteins and polysaccharides) and improve the efficiency of subsequent purification steps. Meanwhile, the low toxicity of EPPS ensures its gentle handling of biological samples without interfering with subsequent biological analysis, ensuring the safety and reliability of the experiment.

3. The compatibility and versatility of EPPS not only make it highly compatible with various commonly used nucleic acid extraction methods, but also effectively support the needs of new high-throughput sequencing platforms, demonstrating strong adaptability and flexibility. In addition, EPPS is commonly used as an electrophoresis buffer, further enhancing the resolution and accuracy of DNA/RNA analysis.

The practical application of EPPS in DNA/RNA separation

1. High quality RNA extraction: EPPS can effectively inhibit the activity of RNA enzymes and reduce RNA degradation, especially when processing tissue samples rich in RNA enzymes. Combined with RNA extraction kit, EPPS can significantly improve the integrity and purity of RNA, providing high-quality templates for subsequent reverse transcription, qRT PCR and other experiments.

2. In the face of biological samples containing a large amount of impurities such as blood cells, cell debris, and proteins, EPPS, with its excellent buffering capacity and low interference characteristics, can assist in the efficient purification of DNA in complex samples. Especially in high-precision applications such as forensic DNA analysis and genetic disease diagnosis, the application of EPPS greatly improves the efficiency and accuracy of DNA extraction.

3. In the era of high-throughput sequencing, the quality of sample preprocessing directly affects the reliability of sequencing results. EPPS, as a key buffer in the construction process of sequencing libraries, has laid a solid foundation for obtaining high-quality sequencing data by optimizing DNA/RNA fragmentation, end repair, and linker ligation steps.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of EPPS and other biological buffering agents. Since its establishment in 2005, it has a history of 20 years, with rich R&D and production experience and professional technical personnel to ensure product quality and after-sales service. If you have any purchasing needs in the near future, please click on the official website to learn more details. The latter can contact me!