Wuhan Desheng Biochemical Technology Co., Ltd

In the early stages of life, every expectant parent yearns to know: Is the baby healthy? In the past, obtaining accurate answers often required invasive methods such as amniocentesis, which brought psychological pressure and intangible risks to pregnant women. Nowadays, with just one tube of pregnant woman's venous blood, it is possible to accurately screen for chromosomal diseases in the fetus - this is the revolutionary change brought about by non-invasive prenatal genetic testing. Behind this change, there is an unknown but crucial 'guardian' - free DNA preservation solution. It is pre installed in a special blood collection tube, guarding the trace fetal code in the blood and providing protection for subsequent precise detection. The 'fetal messenger' in maternal blood In 1997, Professor Lu Yuming discovered the presence of free fetal DNA in the peripheral blood of pregnant women, which completely overturned the pattern of prenatal diagnosis. Originally, during pregnancy, placental trophoblast cells undergo normal apoptosis, releasing short DNA fragments into the maternal bloodstream. These fragments are like "miniature messengers" sent from the fetus, carrying all the genetic information of the fetus. However, these messengers are extremely fragile and rare. In early pregnancy, fetal free DNA accounts for only 5% to 10% of the total free DNA in maternal plasma. What's even more concerning is that after the blood is separated from the body, the mother's white blood cells will gradually lyse, releasing a large amount of maternal genomic DNA, which quickly overwhelms these weak fetal signals like a surging tide. If there are no special protective measures, the sample will be "distorted" just a few hours after blood drawing, and cannot truly reflect the chromosomal status of the fetus. Three major functions, precise protection It is in this context that specialized blood collection tubes containing free DNA preservation solutions have emerged. It is not just an ordinary vacuum tube, but a "mobile laboratory" that integrates multiple technological contents. This preservation solution plays three key roles in non-invasive screening: 1. Stabilize blood cells and prevent interference. The preservation solution contains special cell metabolism inhibitors that can quickly penetrate into blood cells, "immobilizing" these nucleated cells from the source and preventing them from rupturing and releasing genomic DNA during transportation. This is like maintaining order in a noisy venue, ensuring that only the original weak signals can be heard, thereby ensuring the purity of the detection background. 2. Protect DNA and resist degradation. There are naturally occurring nucleases in the blood that can cleave free DNA like scissors. The anti nuclease components in the preservation solution can effectively inhibit the activity of these enzymes, like putting a layer of "protective clothing" on precious fetal DNA to protect them from degradation. 3. Transport at room temperature, breaking down barriers. Traditional testing often requires low-temperature cold chain transportation, which is costly and prone to chain breakage. High quality free DNA storage tubes can achieve stable storage at room temperature for up to 3 to 14 days, which means that pregnant women in remote areas can also enjoy testing services that are homogeneous with those in first tier cities. From a tube of blood to a report The role of free DNA preservation solution in non-invasive screening runs through the entire detection process, and every step cannot be separated from its protection: 1. Sample collection and transportation. When nurses use blood collection tubes containing preservation solution to extract blood from pregnant women, protection is immediately activated. The preservation solution quickly mixes into the blood, stabilizing cells and inhibiting nucleases. Subsequently, the sample can be sent to a testing center thousands of miles away at room temperature, without the need for cold chain and without fear of delay. 2. Plasma separation. After receiving the samples, the laboratory separates the plasma through gradient centrifugation. Thanks to the protection of the preservation solution, the original proportion of fetal free DNA is still maintained in the plasma at this time. Without this layer of protection, the separated plasma may have already been contaminated by maternal background DNA, and the test results would be meaningless. 3. Detection and analysis. Extract free DNA from plasma and perform sequencing and analysis. Clinical data shows that samples protected by high-quality preservation tubes have a detection sensitivity and specificity of over 99% for the three most common chromosomal aneuploid diseases - Down syndrome, Edwards syndrome, and Patao syndrome. Hubei Xindesheng Material Technology Co., Ltd. can supply high-quality DNA preservation solution to accurately assist in non-invasive screening production! If you have any purchasing needs in the near future, please feel free to contact me or click on the official website of Desheng to learn more details!  

Early screening of tumors, as the name suggests, refers to the detection of early or even very early malignant tumors in healthy or high-risk populations. Traditional screening methods, such as imaging examinations, are often difficult to characterize small lesions; As the gold standard for diagnosis, tissue biopsy cannot be used as a large-scale screening method due to its invasiveness. It is in this context that liquid biopsy technology has entered the clinical field, and free DNA preservation solution is the basic support to ensure the practical application of this technology. The tumor code in the blood Circulating free DNA refers to highly fragmented DNA fragments that are free outside of cells in the blood. In a healthy human body, it mainly comes from normal cells undergoing aging and apoptosis. But when a tumor occurs, the situation changes. Tumor cells have a fast proliferation rate and high apoptosis rate, constantly releasing their DNA into the bloodstream. This free DNA from the tumor is called circulating tumor DNA. This type of tumor DNA carries genetic variation information consistent with the primary tumor, including mutations, methylation, copy number variations, etc. In theory, with just one tube of blood drawn, signals from tumors throughout the body can be captured. However, this ideal state faces a serious challenge in reality: the tumor DNA content in the blood is extremely low. Especially in early cancer patients, tumor DNA accounts for only a very small proportion of total free DNA in plasma, sometimes even less than one thousandth. To extract these trace amounts of information from the vast background of blood cells, sample quality becomes the first step in determining success or failure. The two major threats after leaving the body After blood is separated from the body, the sample is not stationary. If there is no intervention, two problems will quickly occur. One is the instability of blood cells. After being isolated, nucleated white blood cells in the blood gradually undergo apoptosis or lysis, releasing a large amount of genomic DNA. These DNA do not come from tumors. When they flood into the plasma, they dilute the already extremely weak tumor signals like a flood. The second is the degradation effect of nucleases. There are naturally occurring nucleases in plasma, whose function is to clear free nucleic acids, which can lead to further fragmentation or even disappearance of valuable tumor DNA fragments. Ordinary blood collection tubes are unable to address these two issues. In traditional EDTA tubes, blood must undergo plasma separation within a few hours, otherwise the sample will 'deteriorate'. For tumor early screening projects that require centralized testing or remote testing, this time window is obviously difficult to meet practical needs. Triple function of preservation solution The free DNA preservation solution is designed to address the aforementioned issues. It is pre sprayed on the inner wall of a dedicated blood collection tube and starts working from the moment blood flows into the tube. Its primary function is to stabilize blood cells. The preservation solution contains special cell metabolism inhibitors that can quickly act on white blood cells, preventing their rupture and release of genomic DNA. This is like closing the door to pollution at the source, ensuring that only the original free DNA in the blood is retained in the plasma. Secondly, it is to protect the existing DNA. The anti nuclease components in the preservation solution can inhibit the activity of nucleases, providing a layer of "protection" for free DNA to prevent its degradation. The third is to provide a transportation window. High quality preservation solution can keep samples stable at room temperature for up to 3 to 14 days. This means that the geographical distance between sampling points and detection centers is no longer a barrier, and remote areas can also enjoy detection services that are homogeneous with those in central cities. Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives. Since its establishment in 2005, it has a history of more than 20 years and has rich production experience. In addition to supplying high-quality free DNA preservation solutions, it can also provide a wide range of products such as serum separation gel, coagulant, heparin sodium, heparin lithium, silicification agent, etc., to meet the one-stop procurement needs of blood collection tube manufacturers! If you are interested, click on the official website to learn more details!  

As we approach the opening of the 2026 Xiamen CACLP (China International Laboratory Medicine and Blood Transfusion Instrument Reagent Expo), this heavyweight event for the IVD industry has become a core platform for industry colleagues to exchange technology, connect resources, and discuss cooperation. Hubei Xindesheng has previously announced its participation in the exhibition. This time, we have specially released exhibition reminders and practical guides to greet all new and old partners. We sincerely invite everyone to visit our booth and chat freely. At the same time, we have prepared a full set of travel and exhibition details to help everyone efficiently observe the exhibition and make appointments without worry. This trip is not in vain. 1, The core message is reiterated: when going to an appointment, do not run away or get lost For the convenience of our partners to quickly verify their itinerary, we have once again clarified the core information of this exhibition and Hubei Xindesheng's participation. We suggest that you collect and keep it to avoid delaying your itinerary. The venue for this exhibition is Xiamen International Expo Center. The official exhibition time is from March 21st to March 23rd, the setup time is on March 20th, and the dismantling time is after 4:00 pm on March 23rd. It is recommended that everyone arrange their travel and exhibition time reasonably. The booth of Hubei Xindesheng is located in Hall 4, 4-A3012, with clear and eye-catching signage. We will be waiting for new and old customers to come and have a face-to-face meeting. 2, Comprehensive transportation guide, smooth bus and self driving experience Xiamen International Expo Center has complete transportation facilities and convenient public transportation, suitable for green travel and avoiding congestion. Customers who take public transportation are advised to take the subway directly to the station near the Expo Center. After exiting the station, they can walk to the entrance of the exhibition hall; You can also choose to take the bus route that passes through Xiamen International Expo Center and directly reach the exhibition hall bus stop. After getting off, follow the exhibition signs to quickly enter. For customers who drive to the exhibition hall, there is a dedicated parking lot around the venue. They can plan their route in advance to avoid rush hour traffic. It is recommended to reserve sufficient parking time to avoid delays in entry due to the upcoming event. After arriving at the exhibition hall, go directly to the fourth exhibition hall area and follow the booth number guide to quickly find the 4-A3012 New Desheng booth. 3, Xiamen weather is known early, comfortable travel is more worry free In late March, Xiamen was in early spring with a mild and comfortable climate. During the exhibition, the temperature was generally between 15 ℃ and 23 ℃, with mostly cloudy weather and occasional light rain. The temperature difference between morning and evening was slightly large, and the sea breeze was gentle. It is recommended that all participating colleagues wear "thin inner layer+lightweight jacket" as the main outfit, which is convenient to add or remove clothes at any time and cope with the temperature difference between morning and evening; You can carry a portable umbrella with you, which can handle short-term light rain and block out daytime sunlight. Before traveling, you can check the real-time weather in advance, make corresponding preparations, maintain a comfortable state throughout the journey, and enjoy the exhibition and negotiation with peace of mind. 4, Warm reminder for exhibitors, efficient exhibition viewing with fewer detours There is a large flow of people at the exhibition site. It is recommended that everyone complete the pre registration for the exhibition in advance, prepare relevant documents, and enter quickly without queuing. After entering, you can first receive the exhibition guide map and then locate the exhibition hall to reduce blind search time. The New Desheng booth will display a full range of core products such as blood collection tube additives, biological buffering agents, and new enzyme preparations. The technical team will be on duty throughout the entire process, allowing for face-to-face communication of product requirements, answering application questions, and negotiating long-term cooperation. We suggest that customers who are interested in cooperation can sort out their demand list in advance for more efficient on-site communication. We will also prepare exclusive materials and souvenirs to give back long-term support to everyone. In early spring, on Egret Island, we will attend a grand event together; Old friends, new friends, talk about the future together. Hubei Xindesheng is always grateful for the companionship and trust of all our partners. This gathering in Xiamen is not only an industry exchange, but also a reunion of old friends. We have prepared tea drinks and product plans at booth 4-A3012 in Hall 4, and look forward to discussing cooperation and seeking common development with you face-to-face. See you in Xiamen from March 21st to 23rd!  

With the deepening of neuroscience research, the importance of human astrocytes in central nervous system research is becoming increasingly prominent. This type of cell not only provides structural support for neurons, but also participates in various physiological processes such as synaptic regulation and metabolic coupling, making it an important model for studying the mechanisms of brain diseases and drug development. However, the in vitro culture of astrocytes is extremely sensitive to environmental changes, especially pH fluctuations, which directly affect their growth status and functional performance. How to maintain a stable microenvironment during the cultivation process has become one of the key issues that researchers need to address. Why do astrocytes require a stable buffer system Human astrocytes are metabolically active during in vitro culture, continuously producing metabolites such as CO ₂ and lactate, which can easily lead to a decrease in the pH of the culture medium. The traditional bicarbonate buffer system relies on a CO ₂ incubator to maintain balance, but in open operations such as fluid exchange and microscopic observation, CO ₂ escape can cause a rapid increase in pH and disrupt cell homeostasis. In addition, astrocytes are sensitive to factors such as metal ion concentration and osmotic pressure. If common buffering agents have metal chelation or chemical instability, they may interfere with cell signaling or induce stress responses. Therefore, choosing a buffer that is independent of CO ₂ and chemically stable is of great significance for maintaining the stability of the culture system. The advantages and application value of MOPS buffer MOPS (3-methylpropanesulfonic acid), as a zwitterionic buffer, has an effective buffering range between pH 6.5-7.9, which precisely covers the optimal growth zone of astrocytes. Unlike bicarbonate systems, MOPS can maintain stable buffering capacity under normoxic or low CO ₂ conditions, reducing environmental interference caused by operational or equipment fluctuations. In optimizing the cultivation system, the role of MOPS is mainly reflected in the following aspects: Maintaining pH stability: In high-density culture or long-term experiments, MOPS can effectively neutralize acidic substances produced by cell metabolism, prevent rapid pH drop in the culture medium, and reduce morphological abnormalities or apoptosis of cells due to acid stress. Not affecting ion balance: MOPS has a stable molecular structure and hardly chelates with divalent ions such as Ca ² ⁺ and Mg ² ⁺, ensuring normal cell adhesion, migration, and calcium signaling processes. Good optical transparency: MOPS has no significant absorption in the UV visible region and is suitable for live cell imaging and fluorescence detection, without affecting the collection of experimental data such as high content screening. Optimize the composition of the cultivation system In practical applications, MOPS is usually used in combination with DMEM/MCDB basic culture medium to balance energy supply and trace element balance. Neurospecific supplements such as Glutamax and N2/G-5/B-27, as well as hEGF and appropriate serum, are also added to the system to meet the nutritional needs of astrocytes. The commonly used addition concentration of MOPS is 10-25 mM, which can be adjusted according to the specific needs of the culture system. Reliable raw material supply guarantee In the study of astrocyte culture, a stable culture environment is the foundation for successful experiments. MOPS, as a reliable buffering agent, is providing sustained and stable support for related research. Hubei Xindesheng Material Technology Co., Ltd. has long been dedicated to the research and production of biological buffering agents. Its MOPS raw materials have a purity of over 99%, strict impurity control, and high inter batch stability, making them suitable for fields such as neural cell culture, molecular diagnostics, and biologics. The new production base of the company located in Huanggang, Hubei is about to be put into operation, equipped with fully automatic synthesis and refining production lines. After production, the production capacity of core products such as MOPS will be further increased, providing more stable supply support for scientific research users and biotechnology enterprises.