Application Of Bis Tris 6976-37-0 In Anion Exchange Chromatography

Chemical Structure and Properties of Bis Tris

Bis Tris, with the molecular formula C8H19NO5, combines functional groups from the bis (2-hydroxyethyl) amine family and Tris family in its chemical structure. This unique structure endows Bis Tris with special chemical properties such as high solubility, high stability, low viscosity, and low ionic strength, making it exhibit outstanding application value in the field of biochemistry.
At 25 ℃, the pKa value of Bis Tris is 6.46, which determines its buffering range. According to buffering theory, Bis Tris has good buffering ability between pH 5.8 and 7.2. Within this pH range, it can effectively resist changes in hydrogen ion concentration caused by external factors and maintain the stability of the solution pH.

Bis Tris powder

The principle and pH requirements of anion exchange chromatography

1. Anion exchange chromatography is an important separation technique widely used in biochemical and molecular biology research. In anion exchange chromatography, the stationary phase often carries a positive charge and can interact with negatively charged ions or molecules. The adsorption and desorption behavior of different anions on a stationary phase depends on their charge density and the pH value of the solution.

2. The change in pH value can affect the ionization degree of the analyte, thereby altering their affinity with the stationary phase. Therefore, maintaining a stable pH value is crucial for achieving accurate separation. Bis Tris, as an efficient buffer, can maintain pH stability within the chromatographic column throughout the entire separation process, providing an ideal reaction environment for anion exchange chromatography.

The role of Bis Tris in anion exchange chromatography

1. Provide a stable pH environment

In anion exchange chromatography, Bis Tris acts as a buffer to maintain pH stability within the column throughout the entire separation process. Its suitable buffer range (pH 5.8 to 7.2) covers many common working pH values in anion exchange chromatography. Within this range, the ionization state of the target anions is relatively stable, maintaining a constant distribution coefficient between the stationary phase and the mobile phase, thereby achieving efficient and accurate separation.

2. Improve separation efficiency and resolution

The buffering capacity of Bis Tris is not limited to maintaining pH stability, but can also improve separation efficiency and resolution by reducing the influence of non-specific reactions and interfering substances. In anion exchange chromatography, Bis Tris ensures predictable migration behavior of target anions in the chromatographic column, reduces separation errors caused by pH fluctuations, and improves the quality of chromatographic analysis.

3. Suitable for the separation of various biomolecules

In addition to anions, Bis Tris can also be used for the separation of various biomolecules, such as hemoglobin, proteins, DNA, and RNA. Its excellent buffering capacity and biocompatibility make Bis Tris an important tool in biochemical and molecular biology research. Bis Tris has demonstrated excellent performance in the separation of hemoglobin, protein chromatography, and DNA purification processes.

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