MOPSO 68399-77-9 Biological Buffer Plays An Important Role In Glycated Albumin Assay Kit

Basic characteristics of MOPSO buffer

MOPSO is a zwitterionic buffer belonging to the Good's buffer series. Its Chinese name is 3- (N-morpholino) -2-hydroxypropanesulfonic acid, which usually appears as a white crystalline powder, has good water solubility, and is not easily affected by temperature and other factors. These characteristics make MOPSO an ideal biological buffer, widely used in various biochemical research and diagnostic kits.

MOPSO buffer

The key role of MOPSO in the determination of glycated albumin

1. Stable reaction environment. The determination of glycated albumin requires specific pH conditions. MOPSO, as a buffering agent, can provide a stable and suitable pH environment, ensuring smooth enzymatic and colorimetric reactions. Its pH buffer range precisely covers the conditions required for measuring glycated albumin, avoiding measurement errors caused by pH fluctuations.

2. Reduce interference factors. In the process of measuring glycated albumin, interference from metal ions and other chemical substances can have a negative impact on the results. MOPSO buffer not only does not precipitate with metal ions, but also effectively reduces the interference of other chemical substances, ensuring the accuracy and reliability of the measurement results.

3. Improve reaction efficiency. MOPSO buffer not only provides a suitable pH environment, but also promotes enzymatic reactions through its zwitterionic properties. In the determination of glycated albumin, MOPSO can optimize the activity of proteases, improve the decomposition efficiency of glycated albumin, thereby shortening the reaction time and improving the determination efficiency.

Specific Application of MOPSO in Glycated Albumin Assay Kit

The glycated albumin assay kit usually includes R1 reagent and R2 reagent, where R1 reagent is mainly used for the decomposition of glycated albumin, while R2 reagent is used for color reaction and final concentration determination. MOPSO plays an important role in both of these reagents.

1. MOPSO in R1 reagent

The R1 reagent contains protease, first buffer (i.e. MOPSO buffer), 4-aminoantipyrine, and other auxiliary components. MOPSO buffer not only provides a suitable pH environment for proteases here, but also ensures the stable progress of glycated albumin decomposition reaction through its buffering effect. At the same time, the ionic properties of MOPSO promote the interaction between protease and glycosylated albumin, improving the decomposition efficiency.

2. MOPSO in R2 reagent

The R2 reagent contains components such as fructose amino acid oxidase, peroxidase, second buffer (also MOPSO buffer), and color reagent. At this stage, MOPSO buffer continues to play its role in stabilizing pH, ensuring the smooth progress of the color reaction. At the same time, MOPSO also promotes the interaction between enzymes and substrates through its zwitterionic properties, improving the sensitivity and accuracy of color reactions.

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