Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: 10191-18-1
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 20kg / box
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 5t/week
Appearance: |
White Powder |
Model: |
BES Buffer |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
Appearance: |
White Powder |
Model: |
BES Buffer |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
Buffers play a crucial role in biological science research and chemical pharmaceutical synthesis processes. They can maintain the acidity and alkalinity of the experimental system, ensuring that biological samples and chemical reactions are carried out under optimal conditions. N. N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES buffer), as an efficient biological buffer, is widely used in laboratory research and development as well as chemical and pharmaceutical fields due to its excellent buffering capacity and stability. This article will provide a detailed introduction to the preparation method of BES powder, helping researchers better understand and use this important reagent.
| Chemical Name | N. N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid, BES buffer |
| Molecular formula | C6H15NO5S |
| Molecular weight | 213.25 |
| Melting point | 150-155 °C |
| Density | 1.210 (estimate) |
| Storage conditions | Sealed storage at room temperature in a cool, ventilated, and dry environment |
Basic properties of BES
N. N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES), chemical formula C6H15NO5S, molecular weight 213.25. It is a white crystalline powder that is easily soluble in water and stable at room temperature and pressure. The pH range of BES is 2.5-5.0 (25 ℃, 1M in H2O), the pH range of acid-base indicator color change is 6.4-7.8, and the acidity coefficient (pKa) is 7.1 (25 ℃). These properties make BES an ideal biological buffer, suitable for various biochemical experiments and pharmaceutical synthesis processes.
BES buffer
Preparation method of BES powder
The preparation of BES buffer requires following certain steps and precautions to ensure that the prepared buffer has a stable pH value and buffering capacity. The following is a detailed preparation method:
1. Prepare materials and equipment
BES powder: Prepare an appropriate amount of BES powder according to experimental needs.
Deionized water: used to dissolve BES powder, ensuring no impurities interfere.
Magnetic stirrer: used to accelerate the dissolution and mixing of BES powder.
PH meter: used to measure and adjust the pH value of buffer solution.
Volumetric flask: used for accurately preparing a certain volume of buffer solution.
2. Preparation steps
Weighing BES powder: Accurately weigh an appropriate amount of BES powder based on the required concentration and volume for the experiment.
Dissolve BES powder: Add the weighed BES powder into an appropriate amount of deionized water and stir with a magnetic stirrer until the BES powder is completely dissolved.
Adjust pH value: Use a pH meter to measure the pH value of the buffer solution and adjust it as needed. If it is necessary to increase the pH value, an appropriate amount of NaOH solution can be added; If it is necessary to lower the pH value, an appropriate amount of HCl solution can be added. Pay attention to gradually adding acidic and alkaline solutions during the adjustment process to avoid excessive fluctuations in pH value.
Constant volume: Transfer the pH adjusted buffer solution to a volumetric flask and dilute it to the desired volume with deionized water.
Mixing: Use a magnetic stirrer again to stir and ensure that the buffer solution is evenly mixed.
3. Precautions
Accurate weighing: When weighing BES powder, make sure to use precise weighing tools to avoid errors.
Fully Dissolve: When dissolving BES powder, a magnetic stirrer should be used for thorough stirring to ensure complete dissolution of the powder.
Gradually adjust pH value: When adjusting pH value, it is necessary to gradually add acid-base solution to avoid excessive fluctuations in pH value that affect the buffering capacity of the buffer solution.
Aseptic operation: If aseptic conditions are required for the experiment, aseptic operation should be carried out during the preparation process to avoid contamination.
Product packaging
Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the production of biological buffering agents such as BES. With advanced equipment and excellent technical talents, we provide professional technical support and good after-sales service for our purchasing customers. With nearly 20 years of rich research and development experience, we can meet the different needs of our customers! If you have a recent need to purchase biological buffering agents, please click on the official website of Desheng to learn more details!
