How To Read And Apply The COA Of Biological Buffer MOPS 1132-61-2

Understand the basic information of MOPS

MOPS, The chemical name is 3- (N-morpholine) propanesulfonic acid, which is an N-substituted amino sulfonic acid with a morpholino ring. It usually exists in the form of a white powder, with high hydrophilicity and good water solubility. MOPS is widely used as a buffer in biological research because it has stable buffering ability in the pH range of 6.5-7.9 and does not interact with metal ions in solution, exhibiting significant metal buffer stability.

MOPS powder

Read the key indicators of COA

1. COA is a detailed quality analysis report provided by the manufacturer, which includes various key quality indicators related to MOPS reagents. When reading COA, researchers should focus on the following key indicators:

2. Purity: The purity of MOPS is an important indicator to ensure its performance. Generally, a purity of over 99% is required, and high-purity MOPS can ensure the accuracy and reliability of experimental results.

3. PH range: Ensure that the pH value of MOPS buffer is between 6.5-7.9, which is the optimal range for its buffering effect.

4. Impurity content: Impurities, especially metal ions, may affect the performance of MOPS. Therefore, the COA should indicate the impurity content and control it at a low limit.

5. Production date and shelf life: Ensure that the MOPS buffer used is within its shelf life to avoid affecting experimental results due to reagent failure. For studies that require multiple repeated experiments, it is recommended to use MOPS buffer from the same batch to ensure consistency of experimental results.

Correct use of MOPS buffer solution

According to the COA provided by the manufacturer, it can help users better use MOPS and understand the precautions for use.

1. Solution configuration:

When configuring 10xMOPS buffer solution, 41.8 grams of MOPS powder are required to be dissolved in approximately 700 milliliters of DEPC treated water.

Adjust the pH value to 7.0 using NaOH.

Add 20mL of 1M sodium peroxyacetate treated with DEPC and 20mL of 0.5M ethylenediaminetetraacetic acid (pH 8.0) to the solution. Dilute to 1L and filter through a 0.45 μ m membrane to remove impurities.

2. Attention:

When configuring and using MOPS solution, care should be taken not to directly expose human skin to high-purity reagents, and protective measures should be taken.

The relevant instruments used must be thoroughly cleaned, and the measuring tools used also need to be calibrated in advance.

When storing the pre configured MOPS solution at room temperature, it is necessary to pay attention to sealing and avoiding light. MOPS solution is prone to yellowing when exposed to light. A light yellow liquid does not affect its use, but if the color is too dark, it is not suitable for continued use.

Sterilization treatment: If MOPS needs to be sterilized, please do not use high-pressure sterilization method. Because high-pressure sterilization may produce unknown pale yellow degradation products. It is recommended to use membrane filtration with a pore size of 0.2 microns or smaller for MOPS sterilization.

Product packaging

COA is an important document for understanding and ensuring the quality of MOPS. By carefully reading the key indicators on the COA, researchers can ensure that the MOPS buffer used meets the experimental requirements. Meanwhile, proper configuration and usage methods are also key to ensuring the accuracy of experimental results. MOPS, as an important biological buffering agent, plays an irreplaceable role in biological and biochemical research.