Why Is MOPSO Buffer 68399-77-9 Recommended For Low-temperature Biochemical Work

1, Stability and low-temperature adaptability of MOPSO buffer

1.1 Stability at low temperatures

Under low temperature conditions, traditional buffering agents are often prone to crystallization or precipitation, which affects the stability and accuracy of experiments. MOPSO buffer exhibits amazing low-temperature stability, maintaining a good solution state even at extremely low temperatures and preventing crystallization or precipitation. This characteristic makes MOPSO an ideal choice for low-temperature biochemical experiments, which can greatly reduce errors caused by crystallization or precipitation during the experimental process, and improve the accuracy and reliability of experimental results.

MOPSO buffer

1.2 Wide pH adjustment range

MOPSO buffer has a wide range of pH adjustment and is suitable for experimental needs with various pH values. This feature provides greater flexibility for experiments, especially in biochemical reactions that require precise pH control. MOPSO can easily meet experimental requirements and ensure stable experimental conditions.

2, The protective effect of MOPSO buffer on biomolecules

2.1 Protecting the activity of biomolecules

During low-temperature storage or low-temperature reaction processes, biomolecules such as proteins and nucleic acids are prone to denaturation and inactivation. MOPSO buffer can effectively reduce deactivation caused by temperature changes and protect the activity and stability of biomolecules by maintaining the pH of the solution and providing a suitable ionic environment. This is crucial for samples that require long-term low-temperature storage or low-temperature biochemical experiments.

2.2 Inhibition of Microbial Growth

In low-temperature biochemical experiments, sample preservation and transportation are key steps. MOPSO buffer can effectively inhibit the growth of microorganisms and reduce the risk of sample contamination. This feature provides a safer and sterile environment for experiments, helping to extend the storage time of samples and ensure the accuracy of experimental results.

3, Unique physicochemical properties of MOPSO buffer

3.1 Difficult to penetrate biological membranes

MOPSO, as a zwitterionic buffer, has a molecular size, shape, and charge distribution characteristic that makes it difficult to penetrate biofilms composed of phospholipid bilayers. This characteristic is of great significance in experiments such as cell lysis, protein extraction, and organelle separation. MOPSO can maintain the stability of the intracellular and extracellular environment, reduce the occurrence of non-specific reactions, and improve the specificity and sensitivity of experiments.

3.2 High solubility and good compatibility

MOPSO buffer has good solubility and compatibility, and can be mixed with other commonly used biochemical reagents for easy experimental operation. In biochemical experiments, the mixed use of multiple reagents is common, and the solubility and compatibility of MOPSO make this process simpler and faster, improving experimental efficiency.

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