Purity and Application Value of Biological Buffer 2- (N-morpholine) ethanesulfonic Acid Monohydrate 145224-94-8

In the research of biochemistry and molecular biology, buffering agents play a crucial role. They can stabilize the pH value of the solution and provide the necessary environment for experiments such as cell culture and enzyme reactions. Among them, 2- (N-morpholine) ethanesulfonic acid monohydrate (MES monohydrate, CAS number: 145224-94-8) is an efficient and non-toxic biological buffer, and its purity directly affects the accuracy and reliability of experimental results. This article will elaborate in detail on the purity standards, applications, and preparation of MES monohydrate.

Purity standards and importance

MES monohydrate is commonly used as a buffering agent in biochemical experiments, and its purity is usually required to reach over 99%. High purity MES monohydrate can ensure a stable and accurate pH environment in the solution, avoiding experimental errors caused by impurity interference. In the laboratory, researchers will choose reputable suppliers to ensure that the purchased MES monohydrate meets strict purity standards, thereby guaranteeing the reliability of experimental results.

MES monohydrate powder

Application of MES monohydrate

1. Adjust the pH value

MES monohydrate is mainly used as a biological buffering agent to regulate the pH value of growth media, culture media, and other biochemical experimental systems. Its buffering capacity can maintain a stable pH range of 5.5 to 7.0 for the solution, which is crucial for experiments such as cell culture and enzyme reactions. MES monohydrate can effectively control the acid-base balance of the experimental system and provide a suitable growth environment for cells.

2. Separation and purification

In the field of molecular biology, MES monohydrate serves as an anionic buffer and is suitable for separation and purification techniques such as cation exchange chromatography and hydroxyapatite chromatography. It can combine with cation exchangers to control the pH value during the chromatography process, which helps to efficiently separate and purify target molecules.

3. Protein electrophoresis

In SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), MES monohydrate as a pH buffer can maintain the stability of protein samples and promote the separation and identification of proteins. Its stable pH environment helps to reduce protein degradation and denaturation, and improve the accuracy of electrophoresis results.

4. Fluorescence microscopy and toxin research

MES monohydrate is also suitable for fluorescence microscopy technology and toxin research. In fluorescence microscopy experiments, MES monohydrate can maintain the pH stability of the sample, which helps to better observe the signal of fluorescent markers. In toxin research, MES monohydrate can simulate real physiological environments, improving the accuracy and reliability of experiments.

Preparation of MES monohydrate

The preparation of MES monohydrate mainly includes direct synthesis and indirect synthesis methods. The direct synthesis method obtains the target product through the substitution reaction and hydrolysis steps of morpholine and sulfonyl chloride; The indirect synthesis method uses morpholine derivatives and sulfonates as raw materials to prepare MES monohydrate through esterification or amidation reactions. Although these methods are different, they all require strict control of reaction conditions to ensure the purity and quality of the product.

Product packaging

Conclusion

In summary, 2- (N-morpholine) ethanesulfonic acid monohydrate (CAS number: 145224-94-8), as a high-purity, non-toxic, and biodegradable biological buffering agent, has broad application value in biochemical and molecular biology research. Its stable chemical properties, excellent buffering capacity, and wide applicability make MES monohydrate an indispensable and important reagent in laboratories. When selecting and using MES monohydrate, researchers should pay attention to its purity standards to ensure the accuracy and reliability of experimental results.

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the research and development, production, and sales of biological buffering agents. It has advanced experimental equipment and professional technical personnel. The purity of MES monohydrate and other biological buffering agents produced can reach over 99%, with good buffering effect and affordable price. If you have any purchasing needs in the near future, please click on the official website to learn more details or contact me. Looking forward to your consultation!