What Does The CAS Number 150-25-4 Of BICINE Buffer Mean

The unique value of CAS number

The CAS number, assigned by the Chemical Abstracts Service (CAS) in the United States, is an internationally recognized unique identifier for chemical substances. It is like an "identity card" for the chemical world, ensuring the unified naming and identification of every chemical substance on a global scale. For N, N-dihydroxyethylglycine (BICINE), CAS number 150-25-4 is its unique chemical identity tag, allowing researchers to quickly and accurately obtain all relevant information about the compound.

BICINE powder

Basic properties of BICINE

N. N-dihydroxyethylglycine, abbreviated as BICINE, is a white crystalline powder chemical substance with the molecular formula C6H13NO4 and a molecular weight of 163.17. As a zwitterionic buffering agent, BICINE has a pH buffering range of 7.6 to 9.0 and excellent water solubility and stability. The substituted amino, carboxyl, and two hydroxyl groups in its molecular structure endow BICINE with unique chemical properties, enabling it to play important roles in various biochemical reactions.

The application of BICINE in the fields of biology and medicine

1. Biological buffering agents

BICINE has been widely used in biological experiments due to its stable pH buffering ability. As a biological buffer, BICINE can maintain and stabilize the pH of the liquid required for experiments, providing a suitable environment for biological reactions. In low-temperature biochemical work, BICINE is recommended as the preferred buffer for preparing stable substrate solutions and conducting experiments such as serum guanine enzyme determination.

2. Electrophoretic buffer

BICINE is often used as a buffer solution for capillary electrophoresis. Its zwitterionic properties enable BICINE to effectively maintain electric field stability and improve electrophoretic separation efficiency during electrophoresis. In molecular biology research, the characteristic of BICINE is of great significance for the isolation and analysis of biomolecules such as DNA and RNA.

BICINE's contribution in the field of environmental protection

BICINE not only shines in the fields of biology and medicine, but also demonstrates its unique charm in the field of environmental protection. As a water-soluble amino acid analogue, BICINE has excellent chelating properties and can efficiently chelate heavy metal ions such as Cu, Cd, Pb, etc. Therefore, BICINE is often used as a leaching solution for the remediation of heavy metal contaminated soil. Under neutral and acidic conditions, BICINE has excellent elution ability for heavy metal ions, which can efficiently remove heavy metal ions from polluted soil while avoiding the loss of plant nutrients such as calcium and magnesium in the soil, achieving a win-win situation for environmental protection and ecology.

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