The CAS Number Of The Biological Buffer 2-cyclohexylamineethanesulfonic Acid CHES Is Revealed

In the field of biological sciences, buffering agents are indispensable experimental tools that play an important role in maintaining stable experimental environments and protecting the activity of biological samples. Among them, 2-cyclohexylamineethanesulfonic acid (CHES) is widely used as an efficient and multifunctional biological buffering agent in biological and biochemical experiments. This article will delve into the CAS number of CHES and provide a detailed introduction to its characteristics, applications, and importance.

1, CAS number resolution for CHES

The CAS number, also known as the Chemical Abstracts Service number, is a unique numerical identification number developed by the Chemical Abstracts Service for chemical substances in the United States. For CHES, its CAS number is 103-47-9, which is not only a symbol of its identity, but also an essential information for researchers in literature search, material purchase, and experimental records.

CHES powder

2, The physicochemical properties of CHES

2.1 Appearance and solubility. CHES, The full name is 2- (N-cyclohexylamino) ethanesulfonic acid, which is a white crystalline powder. At room temperature, it is highly soluble in water, which makes CHES easy to operate when preparing buffer solutions and can quickly reach the desired concentration.

2.2 Stability and pH range. CHES exhibits high chemical stability over a wide range of pH values, particularly in the pH range of 9.3-9.7, where its buffering capacity is particularly outstanding. This characteristic makes CHES an ideal choice for experiments under alkaline conditions, which can effectively maintain the pH stability of the solution and avoid deviations in experimental results.

3, The biological applications of CHES

3.1 Maintain the stability of the intracellular environment. In cell culture experiments, cells are extremely sensitive to changes in the pH value of the environment. CHES, as a biological buffer, can simulate and maintain the pH stability of the intracellular environment, thereby protecting cells from external environmental fluctuations, prolonging cell survival time, and enhancing cell proliferation ability.

3.2 Ion buffering and cell protection. CHES not only has ion buffering ability to maintain the balance of ions inside and outside the cell, but also exhibits a protective effect on the cell. When dealing with adverse conditions such as oxidative stress, CHES can reduce cell damage and ensure the normal physiological function of cells.

3.2 Biological sample processing and simulation. When processing biological samples such as blood and urine, CHES buffer can protect the bioactive substances in the samples and prevent them from becoming inactive or degrading during the processing. In addition, CHES can also be used to simulate the intracellular environment in vitro, providing an experimental platform close to physiological conditions for studying enzyme catalyzed reactions, protein functions, and more.

3.4 Electrophoresis and protein crystallization. In molecular biology experiments, CHES is often used as an electrophoresis buffer to assist in the stable migration of biomolecules such as proteins and DNA in an electric field, thereby improving the resolution and accuracy of experiments. Meanwhile, CHES is also suitable for protein crystallization experiments, which helps to obtain high-quality and high-purity protein crystals.

4, Configuration and usage precautions of CHES

4.1 Configuration method: The configuration of CHES buffer solution is relatively simple. The general steps include preparing the required reagent, weighing CHES powder, dissolving in ultrapure water, adjusting the pH value and fixing the volume. During the configuration process, it is important to use clean containers and tools to avoid contamination; Meanwhile, the concentration and pH value of the buffer solution should be adjusted according to the experimental requirements.

4.2 Precautions for use: Although CHES has many advantages, attention should still be paid to its irritability during use. Wear protective goggles and gloves during operation to avoid direct contact with the skin or inhalation of its powder. In addition, the configured CHES buffer should be stored in a dry, sealed, cool, and dark environment to ensure its stability and effectiveness.

Product packaging

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