What Is The Synthesis Of 3-Morpholinopropanesulfonicacid MOPS Buffer 1132-61-2

In the vast field of biomedical research, immunohistochemical staining technology has become an important tool for revealing the mysteries of life with its unique charm. This technology, with its high specificity and sensitivity, accurately depicts the distribution of proteins within cells and tissues. In this precise exploration journey, 3-methylpropanesulfonic acid (MOPS buffer)  plays an indispensable role with its excellent buffering performance and stability, becoming a trusted partner for scientists.

Basic characteristics of MOPS buffer solution

MOPS, 3-morpholinopropane sulfonic acid is a commonly used biological buffering agent with stable chemical properties, good pH stability, and inertness to various chemical reagents and enzymes. At room temperature, MOPS is a white powder with strong hydrophilicity and good water solubility. After dissolution, it can form a colorless and transparent aqueous solution. The pKa value of MOPS is suitable for the pH requirements of various biological experiments, and has characteristics such as low salt effect, difficulty in penetrating biological membranes, and no formation of complexes or precipitates with metal ions. Therefore, it is widely used in fields such as immunohistochemistry staining.

MOPS powder

The specific application of MOPS buffer in immunohistochemical staining

1. Maintain a stable acid-base environment

During the immunohistochemical staining process, biological molecules such as proteins and antigens in cells or tissues are highly sensitive to acidic and alkaline environments. MOPS buffer can effectively maintain the required acidity and alkalinity for experiments, ensuring the smooth reaction between antigens and antibodies, thereby improving the accuracy and reliability of staining.

2. Suppress interfering factors

During the immunohistochemical staining process, factors such as ion strength and ascorbic acid may interfere with the binding of antigens and antibodies, leading to inaccurate staining results. MOPS buffer, with its excellent anti-interference ability, can effectively suppress the influence of these interfering factors and ensure the smooth progress of the staining process.

3. Improve cell compatibility

During the immunohistochemical staining process, damage or death of cells or tissues may affect the experimental results. MOPS buffer, due to its good cell compatibility, can minimize damage to cells or tissues, protect the integrity and antigenicity of samples.

4. Optimize the dyeing effect

During the staining process, MOPS buffer can also provide a suitable pH and ionic strength environment, promote the binding of dyes and proteins, and improve the sensitivity and specificity of staining. By optimizing staining conditions, MOPS buffer can ensure effective staining and accurate visualization of target proteins, providing researchers with clearer experimental results.

The advantages of MOPS buffer in immunohistochemical staining

1. Improve experimental accuracy: MOPS buffer significantly improves the accuracy of immunohistochemical staining by maintaining a stable acid-base environment, inhibiting interfering factors, and enhancing cell compatibility.

2. Simplify the experimental process: The application of MOPS buffer simplifies the experimental process of immunohistochemical staining. Due to its excellent buffering performance and stability, researchers can obtain stable experimental results without frequently adjusting experimental conditions.

3. Expand application scope: The widespread use of MOPS buffer in immunohistochemical staining is not limited to specific types of cell or tissue samples. Due to its excellent performance and stability, MOPS buffer can be applied to various types of biological sample staining, providing researchers with more research options.

Product packaging

In summary, the MOPS buffer solution of 3-morpholinopropanesulfonic acid plays a crucial role in immunohistochemical staining. It is a promoter of experimental accuracy, a simplification of processes, and a broadening of application scope. Hubei Xindesheng Material Technology Co., Ltd., as a professional manufacturer of biological buffering agents, is committed to providing high-quality MOPS buffer products. We have stable production processes, high-purity products, and affordable prices, and promise high-quality after-sales service. We look forward to working with you!