Is BIS-TRIS 6976-37-0 a derivative of TRIS (Trimethylaminomethane)?

Basic understanding of BIS-TRIS

BIS-TRIS, Bis (2-hydroxyethyl) amino (trihydroxymethyl) methane is a more complex organic compound with a molecular weight of 209.24 g/mol. Structurally, BIS-TRIS introduces additional hydroxyethyl groups on the basis of TRIS. Therefore, from a chemical structure perspective, BIS-TRIS can indeed be regarded as a derivative of TRIS, which has obtained new functions and uses through structural modification.

BIS-TRIS powder

Properties of BIS-TRIS

2.1 Solubility and Stability

BIS-TRIS has high solubility and stability, can be stored stably at room temperature, and is not easily decomposed. This characteristic makes BIS-TRIS more convenient in preparation and storage, while also ensuring its reliability in experiments. In addition, BIS-TRIS has low viscosity and ionic strength, which are crucial for maintaining protein stability and activity.

2.2 Acid base regulation ability

BIS-TRIS, as a synthetic buffer, has good acid-base regulation ability and a wide pH adjustment range, making it suitable for various experimental conditions, especially playing an important role in pH sensitive biochemical experiments.

Application of BIS-TRIS in scientific research field

3.1 Protein research

BIS-TRIS is widely used in protein research to prepare protein buffer solutions. By adjusting the pH value of the solution, BIS-TRIS can maintain the stability and activity of proteins, thereby ensuring the accuracy of experimental results. In addition, BIS-TRIS can also coordinate with various metal ions to form stable complexes, which are of great significance in protein structure analysis and functional research.

3.2 Electrophoretic Techniques

In electrophoresis experiments, BIS-TRIS can provide an ideal buffer environment to help separate proteins and detect their relative molecular weight. In addition, BIS-TRIS is also suitable for DNA and RNA electrophoresis experiments to detect the length and concentration of target nucleic acids.

3.3 Cell culture

In the field of cell culture, Bis Tris propane buffer is used for the preparation of cell culture media. This buffer can maintain a stable pH value during cell culture and provide a suitable environment for cell growth. This is of great significance for cell biology research.

In summary, BIS-TRIS, as a derivative of TRIS, has demonstrated special charm and wide application value in the fields of biochemistry and molecular biology. Its excellent solubility, stability, and acid-base regulation ability make BIS-TRIS an ideal choice in many experiments.

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