Bis (2-hydroxyethyl) amino (trihydroxymethyl) methane BIS-TRIS 6976-37-0 for detecting creatine kinase

In the vast field of biochemical research, buffering agents play a crucial role in maintaining the acid-base balance of reaction systems, and their importance is self-evident. Among them, bis (2-hydroxyethyl) amino (trihydroxymethyl) methane (BIS-TRIS) has shown good advantages in creatine kinase (CK) detection due to its special physical and chemical properties and wide application potential. Let's talk about it in detail below.

Properties of BIS-TRIS

BIS-TRIS, As a widely used biological buffering agent, it has good buffering capacity and can maintain a stable acid-base environment in biochemical experiments. In addition, the structure of BIS-TRIS is similar to TRIS, but its dissociation constant only undergoes slight changes when the temperature changes. This stability is crucial for experiments that require pH control. In addition, BIS-TRIS also has good hydrophilicity and biocompatibility, which can tightly bind with enzyme molecules and improve enzyme stability and activity.

BIS-TRIS powder

Preparation method of BIS-TRIS

The preparation process of BIS-TRIS is rigorous and precise. Usually, under specific reaction conditions, high-purity BIS-TRIS crystals can only be obtained by heating, stirring, adding relevant raw materials, and undergoing long-term reaction and dehydration treatment. This process not only requires strict control of reaction temperature and time, but also requires fine filtration and purification of the reaction solution to ensure the quality and purity of the product.

Application of BIS-TRIS in creatine kinase detection

1. Provide a stable pH environment

The activity of creatine kinase is significantly affected by pH value, and excessively high or low pH can lead to a decrease or even inactivation of enzyme activity. BIS-TRIS, as a wide range buffer, can maintain stable buffering capacity under different pH conditions, providing an ideal reaction environment for the detection of creatine kinase. This stability ensures the smooth progress of enzymatic reactions and improves the reliability and accuracy of detection.

2. Improve the sensitivity and accuracy of detection

In the process of creatine kinase detection, BIS-TRIS can reduce the influence of non-specific reactions and interfering substances, and improve the quality of the detection signal. Meanwhile, its excellent hydrophilicity and biocompatibility enable the enzyme to bind with substrates, improving the efficiency of enzymatic reactions and further enhancing the sensitivity and accuracy of detection. This makes BIS-TRIS an indispensable and important reagent in creatine kinase detection.

3. Excellent stability

BIS-TRIS not only has excellent stability, but is also not easily affected by external factors such as temperature and light. This characteristic has made it widely used in clinical diagnosis and laboratory research. Meanwhile, BIS-TRIS is also easy to store and transport, providing convenience for laboratory personnel.

Product packaging

The Wide Application Prospects of BIS-TRIS

With the continuous development of biochemical technology, the application fields of BIS-TRIS are also constantly expanding. In addition to being used for creatine kinase detection, it is also widely used in the separation and purification of biomolecules such as hemoglobin, protein chromatography, and DNA purification. In addition, BIS-TRIS can also coordinate with various metal ions to form stable complexes, playing an important role in the study of the structure and function of biomolecules.

Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of biological buffering agents such as BIS-TRIS, with good product quality and affordable prices. Our professional technical team can provide excellent customized and after-sales services. If you have any purchasing needs in the near future, please click on the official website for more details or contact me!