Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 25kg / barrel
Delivery Time: 3 days
Payment Terms: L/C, T/T, D/P, Western Union
Supply Ability: 1t / day
CAS: |
29915-38-6 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
TAPS |
CAS: |
29915-38-6 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
TAPS |
TAPS (N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid), as a commonly used biological buffer, occupies an irreplaceable position in DNA and RNA experiments due to its unique buffering range and good water solubility. However, when we prepare TAPS buffer into a solution in practical operation, we sometimes find that the solution becomes turbid. What is the underlying reason behind this? This article will take you on a closer look.
Chemical full name | N-Ethyl-N-(3-sulfopropyl)anilinesodiumsalt |
Product abbreviation | TAPS buffer |
CAS number | 29915-38-6 |
Molecular weight | 243.28 |
Molecular formula | C7H17NO6S |
Storage conditions | Room temperature, protected from light and moisture |
Product advantages | Purity ≥ 99%, good water solubility, stable process |
1, The purity of TAPS powder raw materials is too low
When the purity of TAPS powder raw materials is too low, it may contain more impurities and unreacted raw materials. These impurities may not completely dissolve during the dissolution process, or react with TAPS to form insoluble substances. These insoluble substances will form suspended particles in the solution, causing the solution to become turbid. In addition, if the raw materials contain microorganisms or other pollutants, it may also cause turbidity of the solution during the dissolution process.
TAPS powder
2, High water hardness
Water hardness refers to the content of metal ions such as calcium and magnesium in water. When using water with higher hardness to dissolve TAPS, these metal ions are prone to combine with hydroxide ions under alkaline conditions, forming hydroxide precipitates, resulting in the solution becoming turbid. Therefore, when preparing TAPS solutions, deionized water or distilled water should be used as much as possible to reduce the impact of water hardness on the clarity of the solution.
3, Temperature influence
Temperature is one of the important factors affecting solution stability. In the process of preparing TAPS solution, if the temperature is not properly controlled, it may lead to a decrease in solubility, making it impossible for TAPS to completely dissolve in water, resulting in turbidity. Especially in winter or low temperature environments, the solubility of the solution decreases, making it more prone to turbidity. Therefore, when preparing TAPS solutions, it is important to ensure that the operating environment temperature is appropriate and to avoid operating in low temperature environments as much as possible.
4, Improper pH adjustment
The pH value of TAPS solution has a significant impact on its stability. If the pH value is not adjusted properly, it may lead to hydrolysis or ionization reactions of certain functional groups in TAPS molecules, generating insoluble substances and causing solution turbidity. Therefore, when preparing TAPS solution, a professional pH meter should be used to adjust the pH value, ensuring that the solution pH value is within the appropriate range.
5, Impurity pollution
In the process of preparing TAPS solution, if impure reagents are used or the container is not thoroughly cleaned, impurities may be mixed in, leading to solution turbidity. These impurities may come from the reagent itself, the inner walls of the container, or dust in the air. Therefore, when preparing TAPS solutions, high-purity reagents should be used, and the containers used should be thoroughly cleaned and disinfected to ensure the purity of the solution.
Product packaging
In summary, to ensure the accuracy of the experiment, high-purity TAPS powder raw materials should be used when preparing TAPS buffer solution to avoid turbidity. The quality of the raw materials should be ensured by checking their purity labels or conducting purity tests; During the preparation process, attention should also be paid to using deionized water or distilled water as a solvent to reduce the impact of water hardness on the clarity of the solution. In addition, attention should be paid to controlling experimental conditions such as temperature and pH value to ensure the stability of TAPS buffer in the experiment.
Hubei Xindesheng Material Technology Co., Ltd., as an advantageous manufacturer of TPAS and other biological buffering agents, can provide long-term stable supply of buffering agent powder raw materials with a purity higher than 99%. With professional technical personnel and laboratories, we can provide customized services and technical support for customers. If you have any purchasing needs in the near future, please click on the Desheng official website to learn more details or contact me!