Application of Biological buffer BICINE in Plant Enzyme Extraction CAS 150-25-4

In the field of biotechnology, the extraction and purification of plant enzymes has always been a hot research topic. These enzymes have extensive applications in various industries such as medicine, agriculture, and food. In the process of extracting plant enzymes, a key step is to choose the appropriate buffer. BICINE buffer, as a widely used biological buffer, has shown special advantages in plant enzyme extraction.

Characteristics of BICINE buffer

BICINE buffer has excellent buffering ability and stability, which can maintain a constant ion strength over a wide pH range, providing a stable environment for the extraction of plant enzymes. In addition, BICINE also has good water solubility and biocompatibility, which will not have a negative impact on enzyme activity and stability. These characteristics make BICINE an indispensable tool in plant enzyme extraction.

BICINE buffer

Application of BICINE in Plant Enzyme Extraction

1. Optimize extraction conditions. In the process of plant enzyme extraction, pH value is a crucial parameter. BICINE buffer can accurately adjust the pH value of the solution, ensuring that the enzyme is extracted in a good state. By adjusting the concentration and pH value of BICINE, the extraction conditions can be optimized, and the activity and purity of the enzyme can be improved.

2. Protect enzyme activity. Plant enzymes are easily inactivated during the extraction process due to external environmental influences. BICINE buffer can provide a stable ionic environment, reduce the interaction between enzymes and other substances in the solution, and thus protect enzyme activity. In addition, BICINE can also form hydrogen or ionic bonds with enzyme molecules, enhancing their stability.

3. Improve extraction efficiency. In plant enzyme extraction, extraction efficiency is an important indicator to measure the quality of extraction methods. BICINE buffer can adjust the ion strength and pH value of the solution, making it easier for enzymes to be released from plant tissues. Meanwhile, BICINE can also form complexes with some impurities, reducing their interference and improving extraction efficiency.

4. Simplify subsequent processing. After the extraction of plant enzymes is completed, a series of subsequent treatments such as filtration, centrifugation, dialysis, etc. are required. BICINE buffer has good water solubility and biocompatibility, making these subsequent treatment processes more convenient. In addition, BICINE can also be compatible with some commonly used ion exchange resins or chromatography columns, facilitating further purification.

Examples of BICINE buffer in plant enzyme extraction

Taking cellulase as an example, cellulase is an enzyme widely present in plant cell walls and has important industrial application value. During the extraction process of cellulase, the use of BICINE buffer can significantly improve the extraction efficiency and enzyme activity. Researchers have found that under the same conditions, the activity and purity of cellulase extracted using BICINE buffer are higher than those of other buffer agents. This result fully demonstrates the advantages of BICINE in plant enzyme extraction.

packing of products

The BICINE buffer developed and produced by Desheng not only has high purity and good water solubility, but also has a chloride ion content of less than 0.01%, which has a high cost-effectiveness. In addition to Bicine buffer, there are also dozens of buffering agents such as TRIS and MOPS. Desheng has a history of nearly 20 years since its establishment, with rich R&D and production experience, mature R&D team, and professional laboratory equipment. While providing customized services to customers, it also guarantees after-sales service and provides corresponding technical support. If you have any recent purchasing needs, please click on the Desheng official website to learn more details!