MES buffer used in low concentration plant cell culture medium 4432-31-9

In the field of modern plant biotechnology, cell culture technology has become an important means of studying plant growth and development, genetic improvement, and production of bioactive substances. In this process, the formulation and condition control of the culture medium are crucial for cell growth and differentiation. Especially in low concentration plant cell culture media, how to maintain a stable pH value and appropriate osmotic pressure has become a focus of attention for researchers. This article will provide a detailed introduction to the application and importance of MES buffer in low concentration plant cell culture media.

1, MES buffer: guardian of stable pH value

MES (2-morpholine ethanesulfonic acid), as a commonly used buffer, plays an indispensable role in plant cell culture. In low concentration plant cell culture media, due to the low concentration of nutrients, acidic substances produced by cell metabolism are easily accumulated, leading to a decrease in the pH value of the culture medium. MES buffer can quickly absorb and release hydrogen ions, thereby maintaining the stability of the pH value of the culture medium. This stability is crucial for the normal growth and metabolism of cells, as fluctuations in pH directly affect the permeability of cell membranes, enzyme activity, and regulation of metabolic pathways.

MES buffer

2, MES buffer: the stabilizer of osmotic pressure

In addition to maintaining pH stability, MES buffer affects the osmotic pressure of the culture medium. In low concentration plant cell culture media, the balance of osmotic pressure is equally crucial for cell growth and differentiation. Excessive osmotic pressure can lead to cell dehydration and contraction, while low osmotic pressure can cause cells to absorb water, expand, and even rupture. MES buffer, as an ionic buffer, has a sulfonic acid group in its molecular structure that can form hydrogen bonds with water molecules in the culture medium, thereby regulating the osmotic pressure of the culture medium. This regulatory effect helps to maintain the normal morphology and function of cells.

3, MES buffer: key to improving cell culture efficiency

The application of MES buffer in low concentration plant cell culture medium helps to maintain pH and osmotic pressure stability, and also improves the efficiency of cell culture. On the one hand, stable pH and osmotic pressure provide a suitable growth environment for cells, promoting their metabolic activity and proliferation; On the other hand, MES buffer can also interact with other nutrients, promoting the absorption and utilization of nutrients by cells. The combined effect of these factors significantly improves the cultivation efficiency of low concentration plant cell culture medium, providing strong support for the research and application of plant biotechnology.

4, Application cases of MES buffer

In practical applications, MES buffer has been successfully applied in various low concentration plant cell culture media. For example, in Arabidopsis cell culture, researchers successfully maintained the pH stability of the culture medium and promoted cell growth and differentiation by adding an appropriate amount of MES buffer. In addition, MES buffer has also shown good application effects in cell culture of crops such as tobacco and rice. These successful cases fully demonstrate the importance and practicality of MES buffer in low concentration plant cell culture media.

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Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of MES and other biological buffering agents. Currently, there are dozens of products on sale, and our rich production experience ensures after-sales service. Our professional production team provides technical support and can provide customized services for customers. If you have any purchasing needs in the near future, please click on the official website to learn more details!