Exploring the Mysteries of High purity Biobuffer HEPES CAS 7365-45-9

In the field of life science research, there is an indispensable role that biological buffering agents play. Among numerous biological buffering agents, HEPES buffer (4-hydroxyethylpiperazine ethanesulfonic acid) has become the darling of researchers due to its special properties and wide applications. Next, let's delve deeper into this mysterious and important white powder - HEPES.

1.Basic information of HEPES

HEPES, The full name is 4-hydroxyethylpiperazine ethanesulfonic acid, and its CAS number is 7365-45-9. This substance appears as a pure white crystalline powder in appearance, giving people a fresh and pure feeling. It is precisely this seemingly simple white powder that plays a crucial role in the research of life sciences.

HEPES buffer powder

2 The buffering capacity of HEPES

Maintaining a stable pH value is crucial in biological experiments. HEPES is an excellent pH buffer, with a pH range of 6.8-8.2, which can maintain and stabilize the pH of the experimental liquid well. This characteristic has made HEPES widely used in experiments such as cell culture and protein purification.

In protein purification experiments, researchers will choose appropriate biological buffering agents to extract proteins from complex mixtures. In this process, HEPES has become the preferred choice for researchers most of the time due to its excellent buffering ability and low toxicity to cells. Using HEPES as a buffer can maintain the pH value of the crude extract and provide favorable conditions for subsequent purification steps.

3. Water solubility and stability of HEPES

In addition to excellent buffering ability, HEPES also has good water solubility and stability. This makes HEPES easy to operate and use in experiments. Meanwhile, due to its good stability, it can maintain its buffering ability for a long time, providing stable support for the experiment.

4.The application areas of HEPES

HEPES has a wide range of applications in life science research. In addition to the protein purification experiments mentioned above, HEPES is also commonly used in experiments such as cell culture, enzymatic reactions, and gene transfection. In these experiments, HEPES can effectively maintain the normal physiological state of cells and promote the progress of experiments.
For example, in cell culture experiments, researchers need to place cells in suitable culture media for cultivation. As an important component of the culture medium, HEPES can maintain a stable pH value and provide a favorable environment for cell growth and reproduction.

In addition, in experiments such as enzymatic reactions and gene transfection, HEPES also plays an important role. It can help maintain the stability and activity of enzymes, promote the progress of enzymatic reactions; At the same time, it can also serve as a carrier during gene transfection, helping genes smoothly enter cells for expression.

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5.Precautions for using HEPES

Although HEPES has a wide range of applications and excellent performance in experiments, some precautions still need to be taken during use. Due to the production of hydrogen peroxide when exposed to light, HEPES can be toxic to cultured cells or other biologically active objects. Therefore, when using it, direct sunlight should be avoided as much as possible and operations should be carried out in a dark environment. In addition, when preparing HEPES solution, attention should be paid to its concentration and dosage to avoid adverse effects on the experiment.

HEPES, as an excellent biological buffer, plays an important role in the research of life sciences. Its pure white appearance contains powerful scientific power, providing researchers with stable and reliable experimental support. Desheng specializes in producing bioplastics such as HEPES, with high purity, good buffering ability, and affordable prices. We provide product support for related experiments. If you are also interested in our products, please feel free to contact me!