Functional analysis of chromogenic substrate TOOS 82692-93-1 in adenosine deaminase assay

In the field of modern medical testing, adenosine deaminase (ADA), as a key nucleic acid metabolism enzyme, plays an important role in the cellular immune activity of the body. Its activity assay is not only widely used in the diagnosis and differential diagnosis of liver and gallbladder diseases, but also an important basis for auxiliary diagnosis of diseases such as tuberculosis. In this detection process, the chromogenic substrate N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, also known as TOOS reagent, plays a crucial role. This article will delve into the function of TOOS in adenosine deaminase detection, in order to provide valuable references for researchers and practitioners in related fields.

1, Chemical properties and basic applications of TOOS

TOOS, The full name is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, which is a substance with a unique chemical structure. Its molecular formula is C12H18NNaO4S · 2H2O, and its molecular weight is 331.36. As a new type of Trinder's reagent, TOOS has significant characteristics of high water solubility, high sensitivity, and high stability. These characteristics make TOOS widely used in clinical biochemical testing, especially in multiple testing items such as blood glucose, uric acid, and renal function, where it performs well.

TOOS Powder

2, The Chromogenic Mechanism of TOOS in Adenosine Deaminase Assay

TOOS plays a crucial role as a chromogenic substrate in the measurement of adenosine deaminase. Specifically, ADA catalyzes the deamination of adenine nucleoside to hypoxanthine nucleoside, which is then converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Under the action of xanthine oxidase (XOD), hypoxanthine is further converted into uric acid and hydrogen peroxide (H2O2).

In this process, the generated hydrogen peroxide becomes the key substance that triggers the color reaction. In the presence of peroxidase (POD), hydrogen peroxide undergoes a coupling reaction with TOOS and aminoantipyrine (4-AA) to produce quinone dyes. The rate of this color reaction is closely related to the content of ADA, so the activity of ADA can be indirectly reflected by measuring the generation rate of quinone dyes.

3, Advantages and Applications of TOOS in Adenosine Deaminase Determination

The method of using TOOS to detect adenosine deaminase has many advantages. This method has high measurement precision and strong anti-interference ability. Can maintain stable color rendering effect and ensure the accuracy of measurement results.

Secondly, TOOS has good color rendering effect and rapid response. After reacting with adenosine deaminase, TOOS can quickly generate stable color products for easy detection and analysis. This feature makes TOOS have broad application prospects in the field of rapid detection.

In addition, TOOS has a high molar absorbance and is suitable for trace measurements. This enables TOOS to accurately measure extremely low levels of adenosine deaminase in serum, providing strong support for early detection and diagnosis of related diseases.

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