The specific application of the chromogenic substrate HDAOS 82692-88-4 in enzyme activity detection

In the fields of biochemistry and medical diagnosis, enzyme activity detection is a key technology for revealing the mysteries of biological metabolism and disease states. The chromogenic substrate N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium salt (HDAOS reagent) exhibits significant advantages in enzyme activity detection, especially in low enzyme activity detection, due to its unique structure and superior properties.

HDAOS, as a highly water-soluble aniline derivative, is an ideal choice for enzyme activity detection due to its characteristics. The high stability ensures that it is not easily deteriorated during storage and use, providing reliable guarantees for long-term monitoring and repeated experiments. High sensitivity means that even with weak enzyme activity, HDAOS can capture subtle differences in color changes and achieve accurate measurement. In addition, its high selectivity towards hydrogen peroxide enables accurate determination of target substances in complex biological samples, avoiding interference from other components and improving detection accuracy.

HDAOS powder

In enzyme activity detection applications, HDAOS serves as a chromogenic substrate, generating colored products through oxidation reactions. The color intensity is directly related to the amount of oxidation products produced by enzyme catalysis, indirectly reflecting the level of enzyme activity. This principle is widely used for the detection of various enzyme activities.

Taking catalase (CAT) as an example, as a key antioxidant enzyme in organisms, CAT catalyzes the decomposition of hydrogen peroxide into harmless water and oxygen. The reaction between HDAOS and hydrogen peroxide under the action of peroxidase (such as horseradish peroxidase HRP) can generate stable purple or blue dyes. By measuring the absorbance of the dye, the concentration of hydrogen peroxide can be accurately calculated to evaluate CAT activity. This method is not only easy and fast to operate, but also has high sensitivity and accuracy, providing a scientific basis for evaluating cellular antioxidant capacity and disease diagnosis.

In addition, HDAOS can also bind to other enzyme systems and indirectly detect the activity of related enzymes by monitoring the production of hydrogen peroxide or other oxidation products during the reaction process. In the glucose oxidase (GOD) system, GOD catalyzes the oxidation of glucose to produce gluconic acid, while releasing hydrogen peroxide. After the reaction between HDAOS and hydrogen peroxide, stable colored products are formed. By measuring their absorbance, the glucose concentration can be indirectly calculated to evaluate GOD activity. This method has important value in diabetes diagnosis, blood glucose monitoring and other fields. It not only improves the detection efficiency, but also provides strong support for disease treatment and monitoring.

In summary, HDAOS has played an important role in enzyme activity detection due to its high stability, sensitivity, and selectivity. It not only improves detection accuracy and efficiency, but also provides powerful tools for scientific research and clinical diagnosis, promoting the development of biochemistry and medical diagnosis fields.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of the new trinder's reagents. Currently, in addition to HDAOS powder, the new trinder's reagents on sale also include TOOS, TOPS, ADOS, ADPS, ALPS, ALPS, DAOS, MADB, MAOS, etc. Desheng has a professional technical team to ensure product quality while providing customized services and excellent after-sales service. If you have any recent purchasing needs, please click on the official website to learn more details or contact me!