The Advantages Of ADPS 82611-88-9 As A Chromogenic Substrate In Enzyme Activity Determination

In modern biochemical experiments, enzyme activity measurement is a key step in understanding enzyme catalytic mechanisms, evaluating enzyme function, and disease diagnosis. The chromogenic substrate N-ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium salt (ADPS) has shown significant advantages in enzyme activity assays as an efficient, convenient, and reliable reagent. This article will explore in detail the application and unique advantages of ADPS in enzyme activity determination.

Basic characteristics of ADPS

ADPS, The full name is N-ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium salt dihydrate, which is a white crystalline powder with high purity and high water solubility. Its molecular formula is C12H18NNaO4S and its molecular weight is 295.33. ADPS, as a commonly used chromogenic substrate for horseradish peroxidase (HRP) or peroxidase (POD), is widely used in biochemical assay kits and diagnostic tests. Its high water solubility makes ADPS easy to prepare into solutions for use, greatly simplifying experimental operations.

ADPS Powder

The working principle of ADPS in enzyme activity determination

ADPS can quickly respond and produce significant color changes in enzyme catalyzed reactions. This color change is based on the process of hydrogen peroxide (H2O2) being catalyzed by peroxidase, and ADPS being oxidized with 4-aminoantipyrine (4-AAP) or other coupling agents to form quinone imine compounds. This color change is directly proportional to the amount of hydrogen peroxide present in the reaction system, indirectly reflecting the level of enzyme activity. By measuring the absorbance of the reaction product, the concentration of hydrogen peroxide can be accurately calculated to evaluate the activity of the enzyme.

The advantages of ADPS in enzyme activity determination

1. High sensitivity and accuracy

ADPS is known for its high sensitivity, which can quickly detect small pH changes and the amount of hydrogen peroxide generated in the reaction system. This high sensitivity enables ADPS to provide accurate and reliable data when measuring enzyme activity. Even at extremely low concentrations of the substance being tested, ADPS can accurately reflect color changes, providing researchers with precise experimental results. In addition, the color change of ADPS is stable and easy to observe, making it convenient for experimenters to quickly judge the experimental results, thereby improving the accuracy and reliability of the experiment.

2. Simple operation process

In experiments, using ADPS can greatly reduce the complexity and difficulty of operations. Simply add ADPS solution to the reaction system and then add a color developer to observe a significant color change. This simple operation process makes the experiment easier to execute, saving the time and energy of the experimenters. In addition, the ease of use and stability of ADPS also reduce the error rate in experiments and improve the reliability of experimental data.

3. Low cost and high efficiency

ADPS exists in powder form, which is easy to store and transport, reducing storage and transportation costs. Meanwhile, due to its high sensitivity and reliability, it can reduce the number of repetitions and waste in experiments, thereby lowering experimental costs. In addition, the use of ADPS can also save time costs as it can quickly provide experimental results and accelerate the experimental process. This makes ADPS one of the preferred chromogenic substrates for many research institutions and enterprises.

Product packaging

As a manufacturer of the new Trinder's reagents, Desheng can produce ADPS and other powder reagents with high purity, good water solubility, and white crystal powder appearance. The company's production process is stable, with small batch differences and a large inventory of spot goods, ensuring fast delivery. If you have any purchasing needs in the near future, you can click on the official website of Desheng to learn more details or contact me!