TOPS 40567-80-4 Detection Principle Uric Acid Concentration Is Determined By Absorbance

In the fields of modern biomedical and clinical diagnosis, N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt (TOPS reagent), as a novel Trinder's colorimetric reagent, is playing an indispensable role in uric acid concentration detection due to its unique chemical structure and properties.

TOPS not only has high water solubility, high sensitivity, and strong stability, but also provides scientific basis for accurate measurement of uric acid concentration through its absorbance based detection principle. This article will delve into the TOPS detection principle, revealing how it determines uric acid concentration through absorbance, and the significant importance of this technology in medical diagnosis and health monitoring.

TOPS powder

1, The outstanding characteristics of TOPS reagents

TOPS, The chemical name is N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt, which is a white crystalline powder with a CAS number of 40567-80-4, a molecular weight of 297.34, and a purity of usually over 99%. Compared with traditional colorimetric reagents, TOPS has significant advantages.

Firstly, its high water solubility makes TOPS more convenient to prepare and use, as it is less prone to precipitation or crystallization, thereby ensuring the accuracy of the detection results. Secondly, the high sensitivity of TOPS means that it can produce significant color reactions even at lower concentrations, improving the sensitivity of detection. Finally, the chemical properties of TOPS are stable and not easily affected by environmental factors, ensuring the long-term validity of the test results.

2, The core of TOPS detection principle

The core of TOPS detection principle lies in its characteristics based on enzymatic reactions and color changes. Under specific reaction conditions, uric acid in the test sample reacts with uricase to produce hydrogen peroxide. Subsequently, hydrogen peroxide undergoes an oxidation-reduction reaction with TOPS under the combined action of 4-aminoantipyrine (4-AAP) and peroxidase (POD), producing a red quinone imine compound. This compound has a significant absorbance at a specific wavelength, and by measuring this absorbance value, the concentration of uric acid in the test sample can be calculated.

3, The relationship between absorbance and uric acid concentration

The key to the TOPS detection principle lies in the linear relationship between absorbance and uric acid concentration. As the concentration of uric acid increases, the amount of hydrogen peroxide generated also increases, leading to an increase in the amount of red quinone imine compounds produced by the reaction with TOPS, resulting in an increase in absorbance values. On the contrary, when the concentration of uric acid decreases, the absorbance value will also decrease accordingly. Therefore, by accurately measuring the absorbance value of the reaction mixture and comparing it with a pre established standard curve, the concentration of uric acid in the sample can be accurately calculated.

4, The advantages of TOPS detection technology

TOPS detection technology has demonstrated many advantages in uric acid concentration detection. Firstly, its high sensitivity and strong stability enable the detection process to respond quickly at extremely low concentrations, improving the accuracy and reliability of the detection. Secondly, TOPS detection is easy to operate and does not require complex sample processing steps, greatly reducing detection time. In addition, TOPS testing has a wide range of applications, not only for the detection of uric acid, but also for the determination of various biochemical indicators such as cholesterol and creatinine, providing strong support for medical diagnosis.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as a high-quality developer and manufacturer of chromogenic substrates, can provide high-quality diagnostic reagent raw materials for in vitro diagnostic kit manufacturers. If you are also interested in TOPS and other new trinder's reagents, please click on the official website to learn more details or contact me!