Widely Used Chromogenic Substrate TOPS 40567-80-4 In In Vitro Diagnostic Kits

In modern biomedical and clinical diagnostic fields, the chromogenic substrate TOPS reagent (N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt) plays an indispensable role as a key component in biochemical detection kits. Its unique chemical structure and properties enable it to exhibit excellent colorimetric performance and stability in various biochemical tests, providing strong support for medical diagnosis.

1, Basic characteristics of TOPS

TOPS, The chemical name is N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt, which is a white crystalline powder with a molecular weight of 297.34 and a CAS number of 40567-80-4. Its purity is usually over 99%. This compound has good water solubility, high sensitivity, and strong stability, which make it excellent in biochemical detection.

TOPS powder

2, Application of TOPS in Uric Acid and Cholesterol Detection

TOPS, as a novel Trinder's colorimetric reagent, has been widely used in the colorimetric determination of uric acid and cholesterol. Through specific enzymatic reactions, TOPS can react with metabolites of uric acid or cholesterol to generate chromogenic products with specific absorption spectra. The absorption spectrum of this chromogenic product can be measured indirectly by a spectrophotometer to determine the concentration of uric acid or cholesterol.

3, The important role of TOPS in renal function testing

The application of TOPS in renal function testing is particularly noteworthy. TOPS is mainly related to creatinine detection, which is a waste product generated during muscle metabolism, filtered by the kidneys and excreted with urine. Therefore, the concentration level of creatinine in the blood can indirectly reflect the filtration function and excretion ability of the kidneys. TOPS, as a chromogenic substrate, interacts with peroxidase (such as POD or HRP), 4-aminoantipyrine (4-AAP) and other reagents in a creatinine detection kit, forming a highly sensitive and stable detection method.

4, Application of TOPS in other biochemical tests

In addition to uric acid, cholesterol, and kidney function testing, TOPS is also widely used in other biochemical tests. For example, TOPS can be used as a water-soluble reagent to determine the content of hydrogen peroxide by enzymatic photometry. The content of hydrogen peroxide is correlated with metabolites such as glucose and triglycerides in serum, so the content of these metabolites can be indirectly obtained by measuring the content of hydrogen peroxide. In addition, TOPS is also used as a clinical kit and method for detecting serum or plasma free fatty acids, as well as a glucose oxidase single liquid assay reagent. These broad application ranges fully demonstrate the important role of TOPS in biochemical detection.

Product packaging

In summary, the chromogenic substrate TOPS plays an important role in various in vitro diagnostic kits due to its unique chemical structure and properties. Its high sensitivity, strong stability, and excellent color rendering performance provide strong support for medical diagnosis. As a high-quality developer and manufacturer of chromogenic substrates, Hubei Xindesheng Material Technology Co., Ltd. can provide high-quality diagnostic reagents for in vitro diagnostic kit manufacturers. If you are also interested in TOPS and other reagents, please click on the official website for more details or contact me!