Application Of TOPS 40567-80-4 In Biochemical And Renal Function Testing

In modern biomedical and clinical diagnostic fields, the chromogenic substrate TOPS reagent(N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt) plays an indispensable role as a key component in biochemical detection kits. Its unique chemical structure and properties enable it to exhibit excellent colorimetric performance and stability in various biochemical tests, providing strong support for medical diagnosis.

1, Basic characteristics of TOPS

TOPS, The chemical name is N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt, which is a white crystalline powder. Its CAS number is 40567-80-4, molecular weight is 297.34, and purity is usually over 99%. TOPS has excellent water solubility, high sensitivity, and strong stability, which make it perform well in biochemical testing. In addition, TOPS needs to be stored in a low-temperature environment away from light and moisture to ensure its long-term effectiveness and stability.

TOPS powder

2, The application scope of TOPS in biochemical testing

TOPS, as a novel Trinder's colorimetric reagent, is widely used in diagnostic testing and biochemical testing. It is mainly used for colorimetric determination of uric acid and cholesterol, and can be used as a water-soluble reagent to determine hydrogen peroxide by enzymatic spectrophotometry. In addition, TOPS is also used as a clinical kit and method for detecting serum or plasma free fatty acids, as well as a glucose oxidase single liquid assay reagent. These broad application ranges fully demonstrate the important role of TOPS in biochemical detection.

3, Application of TOPS in renal function testing

The application of TOPS in renal function testing is particularly noteworthy. TOPS is mainly related to creatinine detection, which is a waste product generated during muscle metabolism, filtered by the kidneys and excreted with urine. Therefore, the concentration level of creatinine in the blood can indirectly reflect the filtration function and excretion ability of the kidneys.

TOPS, as a chromogenic substrate, interacts with peroxidase (such as POD or HRP), 4-aminoantipyrine (4-AAP) and other reagents in a creatinine detection kit, forming a highly sensitive and stable detection method. This method is based on the principles of enzymatic reaction and colorimetric method, and indirectly determines the concentration of creatinine by measuring the absorbance of the reaction mixture.

The specific steps are as follows: Collect blood samples from the patient's body, and separate serum or plasma through centrifugation. Then, mix the serum or plasma with a reaction solution containing TOPS, peroxidase, and 4-AAP reagents. In the presence of hydrogen peroxide, TOPS undergoes an oxidation reaction with 4-AAP under the catalysis of peroxidase, producing quinone imine colored products. The absorption spectrum of this chromogenic product has a specific wavelength range and can usually be measured by a spectrophotometer. By measuring the absorbance of the reaction mixture and comparing it with a pre established standard curve, the concentration of creatinine in the sample can be calculated.

The advantage of TOPS in renal function testing lies in its high sensitivity and stability. This enables the detection process to respond quickly at extremely low concentrations, improving the accuracy and reliability of the detection. By regularly monitoring the concentration level of creatinine in the blood, doctors can timely understand the patient's renal function status and develop more accurate treatment plans.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. can provide high-quality TOPS and other chromogenic substrate, advanced production equipment, and professional technical personnel to ensure product quality, while also providing high-quality customized and after-sales services. If you have any recent purchasing needs, please click on the official website of Desheng to learn more details or contact me!