Application of The New Trinder's Reagent DAOS 83777-30-4 in Biochemical Detection

DAOS,The full name is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium salt, with a chemical formula of C13H20NO6SNa and a molecular weight of 341.35. It is a highly water-soluble aniline derivative with a purity of over 99% and usually exists in the form of a white powder. DAOS reagent,as a novel Trinder's reagent, can undergo oxidation reactions under specific conditions, which makes it highly applicable in biochemical detection.

Application of DAOS in Biochemical Testing

1. Detection of hydrogen peroxide

DAOS performs well in the detection of hydrogen peroxide. In the presence of hydrogen peroxide and peroxidase, DAOS can undergo oxidative coupling reactions with coupling agents such as 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH) to generate stable purple or blue dyes. This reaction not only has high sensitivity, but also strong stability, and can accurately determine the concentration of hydrogen peroxide in the sample. DAOS plays an irreplaceable role as a chromogenic substrate in the detection of substances such as glucose, alcohol, acyl CoA, and cholesterol.

DAOS Reagent

2. Enzyme photometric determination

DAOS has also demonstrated advantages in enzymatic photometric assays. Enzyme photometry is an analytical method based on a combination of enzymatic and colorimetric reactions, widely used for the detection of various biochemical indicators. DAOS, as a chromogenic substrate, is catalyzed by oxidase during enzymatic reactions to produce hydrogen peroxide. Hydrogen peroxide further reacts with DAOS to generate detectable purple or blue dyes. By measuring the absorbance of dyes, the concentration of substrates in the sample can be indirectly determined, thereby achieving quantitative analysis of biochemical indicators.

3. Diagnostic testing and biochemical experiments

DAOS is widely used in various diagnostic tests and biochemical experiments due to its high water solubility and good stability. In clinical medicine, DAOS can be used for the detection of indicators such as blood glucose, blood lipids, and liver function, providing accurate diagnostic basis for doctors. In the field of scientific research, DAOS is also commonly used for the development and optimization of new detection methods, promoting the progress of biomedical research.

The advantages and prospects of DAOS

1. Advantages

High water solubility: DAOS has good water solubility and can quickly dissolve and function in aqueous solutions, improving the sensitivity and accuracy of detection.

Strong stability: The dye formed by DAOS during the reaction process has high stability and is not easily affected by external factors, ensuring the reliability of the detection results.

High sensitivity: DAOS has high detection sensitivity for substances such as hydrogen peroxide, and can detect extremely low concentrations of target substances.

Widely applicable: DAOS can be used not only for the detection of hydrogen peroxide, but also for the detection of various other biochemical indicators, with broad application prospects.

Product packaging

2. Prospects

With the continuous development of biomedical technology, the requirements for biochemical detection reagents are also increasing. DAOS, as a novel Trinder's reagent, will play a more important role in future biochemical detection due to its unique chemical properties and wide application potential. In the future, with the continuous deepening of scientists' research on DAOS, its application fields will be further expanded, providing more accurate detection methods for biomedical research and clinical diagnosis.

Hubei Xindesheng Material Technology Co., Ltd. specializes in the research and production of the new Trinder's reagents. Currently, in addition to DAOS, which has high purity, good water solubility, and high sensitivity, there are also more than ten reagents available for sale, including TOOS, TOPS, ADPS, MAOS, etc. There are many types of products with high selectivity. If you have any recent purchasing needs, please click on the official website to learn more details or contact me!