Methods for improving the stability of chromogenic substrate TOOS reagents 82692-93-1

In the field of biochemical detection, the chromogenic substrate TOOS reagents (chemical name N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, or 3- (N-ethylmethylaniline) -2-hydroxy-propanesulfonic acid sodium salt) is the core chromogenic reagent in enzymatic spectrophotometry, and its stability directly affects the sensitivity and accuracy of detection. Therefore, exploring how to effectively maintain the stability of TOOS reagents has become an urgent problem to be solved in scientific research and production practice.

Application of TOOS reagents

The high molar absorbance of TOOS endows it with excellent color sensitivity, which makes it widely used in both wet chemical (solution) and dry chemical methods. In the dry chemistry method, the TOOS reagent, coupled 4-AAP, and required enzymes (such as HRP) are fixed on the membrane carrier. When the sample is added dropwise, H2O2 is generated and new ecological oxygen is released, thereby oxidizing the chromogenic substrate. By measuring the amount of reaction products, the target component can be indirectly determined. However, it should be noted that in dry chemical methods, the stability of TOOS and enzymes is poor due to factors such as temperature, pressure, and light. Similarly, in wet chemical methods, enzymes are also prone to deactivation when they are in liquid state or at low concentrations. Therefore, improving the stability of TOOS reagents is very important.

TOOS reagents

Properly store TOOS reagents

A proper storage method is the first step to improve the stability of TOOS reagents. This is because TOOS reagents are prone to oxidation when stored improperly. Once oxidized in advance, it will directly reduce their stability and color rendering ability, and also directly affect the accuracy of detection results. Taking the TOOS reagent from Desheng as an example, in order to maintain its stability, the reagent is in the form of a solid powder and stored at low temperature in a sealed and opaque bottle. This storage method effectively isolates air and light, prevents TOOS from being slowly oxidized by air due to long-term storage, and also avoids spoilage caused by high temperature or light.

Methods for improving the stability of chromogenic substrate TOOS

1. Adding protective agents: In order to improve the stability of TOOS, it can be achieved by adding protective agents. For example, surfactants with alkyl groups with 8-16 carbon atoms and flavonoid pigments can serve as protectants for TOOS. However, the formula for this method is complex and may interfere with testing. In addition, although various enzymes (such as ALT AST, LDH, ALP, CK can improve enzyme stability in aqueous solutions or serum matrices, but the effect is not significant in dry chemical test strips.

2. Choose a protective agent with stronger reducibility: Another method is to add a protective agent with stronger reducibility than TOOS. However, the protective agent in this method usually contains primary amino groups, which can easily cause non-specific reactions. Some researchers have attempted to add sodium citrate, which has a reducing effect, and found that it has a certain antioxidant effect on TOOS.

3. Ready to use: Based on Desheng's experience, in order to ensure the stability of TOOS and the accuracy of detection results, it is recommended to prepare TOOS reagents temporarily during use to avoid long-term storage. This not only reduces oxidation problems caused by improper storage, but also ensures that the reagent is used in its optimal state.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. specializes in producing TOOS and other the new trinder's reagent raw material powders. The product has high purity and can ensure a white powder appearance. It has good water solubility and is easy to prepare and use. If you have any purchasing needs in the near future, please click on the Desheng official website to learn more details or contact me!