Application of The New Trinder's Reagent TOPS 40567-80-4 in Cholesterol Colorimetric Determination

With the rapid development of modern medicine and biotechnology, fast and accurate detection methods play a crucial role in clinical medicine, biochemical analysis, and other fields. Among numerous detection reagents, the new Trinder's reagent TOPS plays an important role in cholesterol colorimetric determination due to its advantages of high sensitivity, good water solubility, and strong process stability. This article will provide a detailed introduction to the application and advantages of TOPS reagents in cholesterol colorimetric determination.

Introduction to TOPS reagents

TOPS, The full name is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt, which appears as a white powder. It is a water-soluble reagent used for enzymatic photometric determination of hydrogen peroxide and is widely used in diagnostic testing and biochemical experiments.

TOPS reagent

Application of TOPS reagent in colorimetric determination of cholesterol

Cholesterol, as an important lipid substance in the human body, plays an important role in maintaining cell membrane structure and other aspects. However, high cholesterol levels can increase the risk of cardiovascular diseases. Therefore, accurate measurement of cholesterol levels is of great significance for regulating physical indicators. The application of TOPS reagents in cholesterol colorimetric determination provides a convenient detection method for clinical medicine.

1. Detection principle

The detection principle of TOPS reagent in cholesterol colorimetric determination is mainly based on its use as a commonly used colorimetric reagent in biochemical reagent kits, especially as a substrate for peroxidase (HRP or POD). In cholesterol colorimetric assay, in the presence of 4-aminoantipyrine (4-AAP), peroxidase (POD or HRP), and TOPS (as chromogenic substrates), hydrogen peroxide reacts with chromogenic substrates to produce red quinone imine compounds.

The intensity of this color reaction can be measured by absorbance and is directly proportional to the cholesterol content. Therefore, by measuring the absorbance of the reaction system, the concentration of cholesterol in the sample can be calculated.

2. Advantage analysis

(1) High sensitivity: TOPS reagents can detect very low concentrations of cholesterol, improving the accuracy of detection.

(2) Easy to operate: The entire detection process is simple and feasible, without the need for complex instruments and professional skills, reducing the difficulty and cost of operation.

(3) Rapid response: TOPS reagents have the characteristic of rapid response and can complete detection tasks in a short period of time.

(4) Good stability: TOPS reagents have good water solubility, high purity, and strong process stability, which can ensure the stability and reliability of detection results.

Product packaging

Storage and precautions for TOPS reagents

When storing TOPS reagents, the following points should be noted:

1. Due to its water-soluble characteristics, TOPS reagents need to be stored in a closed and dry environment to prevent changes in chemical properties due to moisture absorption;

2 TOPS reagents are sensitive to light and need to be packaged in light proof bottles;

3. During the operation process, relevant regulations and operating procedures should be followed to ensure the accuracy of experimental results.

The TOPS reagents and other new Trinder's reagents produced by Hubei Xindesheng Material Technology Co., Ltd. have high purity, good water solubility, stable production process, and small inter batch differences. All indicators meet relevant standards. If you have any purchasing needs recently, please feel free to contact me or click on the official website to learn more details!