The application of DAOS 83777-30-4 in the detection of triglycerides and high-density lipoprotein

In the fields of biochemistry and medical diagnosis, the detection of triglycerides (TG) and high-density lipoprotein (HDL) is crucial for assessing lipid metabolism and cardiovascular disease risk in the human body. DAOS reagent(a chromogenic substrate), as a key reagent in modern biochemical detection, plays an irreplaceable role in the detection of TG and HDL. This article will explore in detail the application principles, methods, and importance of DAOS in TG and HDL detection.

1, Application of DAOS in Triglyceride Detection

Triglycerides are the main storage form of lipids in the body, and their abnormal increase in concentration is closely related to the occurrence of cardiovascular diseases. Therefore, accurate measurement of TG concentration is of great significance for the prevention, diagnosis, and treatment of diseases.

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The application of DAOS in TG detection is based on a series of enzymatic and colorimetric reactions. Firstly, triglycerides are hydrolyzed into glycerol and fatty acids through specific enzymes, and glycerol is further oxidized to glyceraldehyde-3-phosphate (DHAP) and produces hydrogen peroxide (H ₂ O ₂). Under the catalysis of peroxidase, H ₂ O ₂ undergoes coupling reaction with DAOS and 4-aminoantipyrine (4-AAP) to produce colored products. The absorbance of this colored product is directly proportional to the concentration of TG, so the concentration of TG can be quantified by measuring the absorbance.

The application of DAOS in TG detection has the following advantages: firstly, this method has high sensitivity and accuracy, which can meet the needs of clinical detection; Secondly, this method is easy to operate, fast, and suitable for detecting large-scale samples; This method does not require expensive equipment or complex operational skills, and is easy to promote and use in grassroots institutions.

2, Application of DAOS in high-density lipoprotein detection

High density lipoprotein is a beneficial lipoprotein that has the function of transferring cholesterol from peripheral tissues back to the liver for metabolism. Therefore, the increase in HDL concentration is considered one of the important factors in reducing the risk of cardiovascular disease.

The application of DAOS in HDL detection is based on its specific binding ability with HDL. When HDL is combined with DAOS, color changes occur. This color change can be quantified by measuring absorbance to quantify the concentration of HDL. To eliminate interference from other lipoproteins in HDL determination, specific inhibitors or separation methods are usually used to separate HDL from other lipoproteins.

The application of DAOS in HDL detection also has many advantages. This method has high specificity and accuracy, and can accurately reflect the concentration of HDL; Secondly, this method is easy to operate, fast, and suitable for routine clinical testing; This method is of great significance for assessing the risk of cardiovascular disease and helps guide disease prevention and treatment.

3, Conclusion

DAOS, as an important chromogenic substrate, plays an irreplaceable role in the detection of triglycerides and high-density lipoprotein. It achieves accurate determination of TG and HDL concentrations through enzymatic and colorimetric reactions, providing strong support for disease prevention, diagnosis, and treatment. With the continuous development of technology, the application of DAOS in the field of biochemical testing will become increasingly widespread, bringing more convenience and possibilities for medical diagnosis and disease treatment.

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Desheng, as an established supplier in the field of chromogenic substrate, we focus on providing high-performance DAOS products to meet the precise needs of various experimental scenarios. We firmly believe that high-quality DAOS not only ensures the accuracy of experimental results, but also helps researchers achieve breakthroughs in basic research and clinical diagnosis. We are committed to providing reliable and strong support for your research journey. If you are interested in purchasing DAOS, we sincerely invite you to contact us, and we are always ready to provide you with services!