99% Tris(Hydroxymethyl)Aminoethane Tromethamine Aminobutanol THAM 77-86-1

All you want to know about TRIS buffer is here

1. What is TRIS buffer?

Tris(hydroxymethyl)aminomethane, or TRIS for short, is called tromethamine or THAM in medical applications. It is an organic compound with the molecular formula (HOCH2)3CNH2. It is widely used in biochemistry and molecular biology as a component of buffer solutions, and its derivatives include TAE and TBE buffers.

TRIS buffer powder

.

2. What is the pH value of TRIS buffer?

Tris buffer is an ideal choice for most biological systems because its pKa value at 25°C is about 8.1 making it an effective buffer in the pH range of 7-9. This pH range is suitable for most biological experiments. This is also the reason why TRIS buffer is widely used.

3. Are Tris buffer and Tris base the same?

The pH value of Tris-base 1M is about 11, and the pH value can be reduced to 7 by adding HCL. But in Tris-Hcl, if you want to lower the pH to 7 you must add NaOH, because tris-Hcl contains hydrochloric acid, so it has a higher ionic strength (HCl + NaOH). But in Tris-base, you only need to add HCl.

4. Why use TRIS for DNA extraction?

Tris is a common biological buffer that can be used in the entire DNA extraction process. Because it is sensitive to pH during DNA extraction. Therefore, in the process of cell lysis, removal of unwanted cell components and precipitation, tris buffer is needed to maintain a stable pH value.

TRIS powder

Advantages of Biological Buffer Tris

TRIS Chinese name: Tris (hydroxymethyl) aminomethane, white crystalline powder in appearance, good water solubility, can also be dissolved with ethyl acetate but the dissolution rate is relatively slow, carbon tetrachloride, copper, aluminum There is a corrosive effect. Tris has a PKA value of 8 at 25°C and a buffer range of 7-9. Common Tris buffers include Tris-HCL buffer, Tris-phosphate buffer and a variety of derivative buffers, such as TBS, TE, etc. TRIS is a very important biological buffer, the CAS number is 77-86-1.

In biochemical research, biological buffers are often used, and there are as many as 20-30 varieties of biological buffers. Biological buffers are widely used in the in vitro diagnostic industry and biochemical experiments. What are the advantages of Tris in biological buffers?

1. Tris has high solubility in water and is inert to the reaction of many enzymes.

2. Tris has a high buffering capacity. It is generally used to stabilize the pH of the reaction system. It has a strong buffering capacity between pH 7.5-9.0.

3. Tris is very alkaline, so this buffer system can be used to configure a wide range of pH buffers ranging from acidic to alkaline.

4. Tris has little interference to the biochemical process and will not react with calcium, magnesium ions and heavy metal ions.

5. Tris buffer is widely used, and plays an important role in the fields of biochemistry, cosmetics, in vitro diagnostics, molecular biology and coatings.

Product packaging

When using Tris buffer, it is also necessary to note that the pH value of Tris is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process. Tris will easily absorb CO2 in the air, and the prepared buffer should be tightly sealed to avoid loss. In addition, Tris will react with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals. It can also act with heavy metals, but it also has an inhibitory effect on some systems.