China Wuhan Desheng Biochemical Technology Co., Ltd Company News

In the fields of biochemistry and molecular biology, enzyme activity detection is the core means of measuring whether biochemical processes in organisms are operating normally. However, in practical operation, there is often a problem of low enzyme activity, which makes it difficult for traditional detection methods to accurately capture these weak signals. In recent years, the chromogenic substrate HDAOS reagent (N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium salt) has emerged in the field of enzyme activity detection, especially in low enzyme activity detection, due to its unique chemical structure and excellent performance. Basic features of HDAOS HDAOS, as a highly water-soluble aniline derivative, has high stability, sensitivity, and selectivity, which enable it to accurately capture weak signals in enzyme activity detection and provide reliable measurement results even when enzyme activity is low. 1. High stability: HDAOS exhibits good stability during storage and use, and is not easily oxidized or decomposed, ensuring the accuracy and reliability of detection results. Even after long-term use and storage, its performance remains stable, which is crucial for long-term monitoring and repeated experiments. 2. High sensitivity: HDAOS reacts rapidly with oxidants such as hydrogen peroxide and can form stable color reactions in a short period of time. This rapid and stable color change provides a solid foundation for enzyme activity detection. Even at low enzyme activity, HDAOS can capture small color changes with its high sensitivity, achieving precise measurement. 3. High selectivity: HDAOS has extremely high selectivity for hydrogen peroxide and can accurately determine the concentration of hydrogen peroxide in complex samples without interference from other molecules. This high selectivity ensures that enzyme activity detection can accurately reflect the activity of the target enzyme, avoiding interference from other enzymes or compounds, and providing accurate measurement results even in the presence of low enzyme activity and interfering substances. Application of HDAOS in Enzyme Activity Detection In enzyme activity detection, HDAOS serves as a chromogenic substrate or indicator, and its basic principle is to undergo an oxidation reaction in the presence of hydrogen peroxide or other oxidants to generate colored products. The color change is directly proportional to the amount of hydrogen peroxide or other oxidation products produced in enzyme catalyzed reactions, indirectly reflecting the level of enzyme activity. 1. Catalase activity detection: Catalase (CAT) is an important antioxidant enzyme in organisms, which can catalyze the decomposition of hydrogen peroxide into water and oxygen, protecting cells from oxidative damage. Using HDAOS as a chromogenic substrate, it reacts with hydrogen peroxide under the catalysis of peroxidase (such as horseradish peroxidase HRP) to generate stable purple or blue dyes. By measuring the absorbance of the reaction product, the concentration of hydrogen peroxide can be calculated to evaluate the activity of catalase. This method is easy to operate, fast, and has high sensitivity and accuracy. 2. Indirect detection of other enzyme activities: HDAOS can also be combined with other enzyme systems to indirectly evaluate related enzyme activities by monitoring changes in hydrogen peroxide or other oxidation products produced during the reaction process. For example, in the glucose oxidase (GOD) system, glucose is oxidized to gluconic acid under GOD catalysis, while producing hydrogen peroxide. Hydrogen peroxide reacts with HDAOS catalyzed by HRP to produce colored products. By measuring the absorbance of the product, the glucose concentration can be calculated, indirectly reflecting the activity of GOD. This method has a broad application prospect in diabetes diagnosis, blood glucose monitoring and other fields. The advantages of HDAOS in low enzyme activity detection 1. High sensitivity capture of weak signals: HDAOS reacts rapidly and stably with oxidants such as hydrogen peroxide, providing a reliable basis for low enzyme activity detection. Even at low enzyme activity, small color changes can be captured with high sensitivity, achieving precise measurement. 2. High selectivity reduces interference: HDAOS's high selectivity towards hydrogen peroxide ensures the accuracy of enzyme activity detection, allowing for accurate determination of hydrogen peroxide concentration even in complex samples without being affected by it. Conclusion In summary, the chromogenic substrate HDAOS exhibits accurate measurement ability in low enzyme activity detection, and its high stability, sensitivity, and selectivity make it outstanding in the field of enzyme activity detection. By combining with other enzyme systems, HDAOS can indirectly evaluate the activity of related enzymes, providing strong support for research and diagnosis in biomedical, environmental monitoring, and food safety fields. With the continuous advancement of technology and the expansion of application fields, HDAOS is expected to play an important role in more fields, providing more accurate and reliable tools for life science research and clinical diagnosis. As a professional manufacturer of chromogenic substrate, Desheng offers a complete range of products, including corresponding TOOS, TOPS, ADOS, ADPS, MAOS, etc., which can provide customers with one-stop purchasing services. Due to 18 years of production experience, the product quality has been widely recognized by the market, so in recent years, we have continuously expanded overseas and sold our products to countries around the world. If you have relevant purchasing intentions, please click on the website for consultation!

Acridine ester is a chemical substance that can be used as a chemiluminescent label. Acridine salts and related compounds have been widely proven to be very useful chemiluminescent labels. Its stability, labeling specificity and detection sensitivity surpass that of radioisotopes. . Under alkaline conditions, NHS is substituted as a leaving group, and the protein forms a stable amide bond with the acridine ester. However, acridinium ester is only a general term for acridinium salt. If subdivided, Desheng can provide 7 different acridinium esters (see the table below) for you to choose from, so what are these 7 acridinium esters? What are the similarities and differences? Let us continue to look down. Magnetic particle chemiluminescence method using acridinium ester 1. Desheng acridine ester types: 1.1 Acridinium Ester No. 0-Acridinium Ester (AE-NHS)【CAS】None【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】N/A【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.2 Acridinium Ester No. 1-Acridinium Ester (DMAE-NHS)【English name】2',6'-dimethyl-4'-(N-succinimidyloxycarbonyl)phenyl-10-methyl-acridinium-9-carboxylate methosulfate[Chinese name] 9-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,6-dimethylphenoxy]carbonyl]-10-methylAcridine Methyl Sulfate【CAS】115853-74-2【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】594.13, C29H26N2O10S【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.3 Acridinium Ester No. 2-Acridinium Ester (Me-DMAE-NHS)【English name】2',6'-Dimethylcarbonylphenyl 10-Methyl-9-acridinecarboxylate 4'-NHS Ester Triflate[Chinese name] 2',6'-Dimethylcarbonylphenyl 10-methyl-9-acridine carboxylate 4'-NHS ester trifluoromethanesulfonate【CAS】None (115853-74-2, DMAE-NHS analogue)【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】632.56, C29H23F3N2O9S【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.4 Acridinium Ester No. 3-Acridinium Ester (NSP-DMAE-NHS)【English name】2',6'-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'- NHS Ester[Chinese name] 9-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,6-dimethylphenoxy]carbonyl]-10-( 3-Sulfopropyl)-acridine inner salt【CAS】194357-64-7【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】590.6, C30H26N2O9S【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.5 Acridine ester No. 4-acridine salt (NSP-SA)[English name] 3-[9-(((3-(carboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonateinner salt[Chinese name] Acridine salt (NSP-SA)【CAS】211106-69-0【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】584.66, C28H28N2O8S2【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.6 Acridine ester No. 5-acridine salt (NSP-SA-NHS)[English name] 3-[9-(((3-(N-succinimidyloxycarboxypropyl)[4-methxylphenyl]sulfonyl)amine)carboxyl]-10-acridiniumyl)-1-propanesulfonateinner salt[Chinese name] Acridine salt (NSP-SA-NHS)【CAS】199293-83-9【Appearance】Yellow solid or powder【Purity】≥98%【Molecular Weight】681.73, C32H31N3O10S2【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. 1.7 Acridine ester No. 6-acridinium hydrazide (NSP-SA-ADH)【CAS】None【Appearance】Yellow solid or powder【Purity】≥98%【Molecular weight】740.85, C34H40N6O9S2【Storage conditions】-20℃, avoid light[Transportation Conditions] It can be transported at room temperature (20-25°C) within 48 hours, and it needs to be transported at -20°C for more than 48 hours. two. The structural difference of acridine esters Six kinds of acridine esters, of which Nos. 1 to 3 are acridine esters; Nos. 4 to 6 are acridine sulfonamide lactates; Nos. 1 to 4 are NHS active esters; No. 5 is acridine carboxylic acid with carboxyl group; 6 The number is acridine hydrazide, which contains free amino groups. three. Hydrolysis resistance and stabilityTraditional acridinium ester No. 0 (AE-NHS) and No. 1 to 3 have been modified in their structure to increase steric hindrance and enhance hydrolysis resistance. No. 0 is only stable in acidic solutions. When the pH value is greater than 6.3, it is easy to hydrolyze, while No. 1 to 3 are not. For example, at room temperature, it is very good in PB buffer with pH 7.0.It is stable. After 16 days, the luminescence activity is only reduced by 3.6%.No. 4 to No. 6 is the introduction of sulfonamide groups, and its stability is higher than that of acridine esters (No. 0 to No. 3). The reason is that the C-N bond and C-O bond are more stable.The bond level is different, the C-N bond is larger than the C-O bond. Acridine amide compounds (No. 4～6) have stronger resistance to hydrolysis than acridine esters (No. 0～3).Things. No. 4 to No. 6 are stable in acidic solution (pH

The China International Laboratory Medicine and Blood Transfusion Instrument Reagent Expo (CACLP) in 2025 is about to kick off. CACLP is the annual event of the in vitro diagnostics (IVD) industry. How can IVD professionals gain something from this grand event? It is a question worth pondering deeply. Here are some practical methods and strategies for participating in exhibitions to help you achieve success in 2025CACLP. 1, Accurately locate and clarify key visiting areas Take some time to carefully browse the exhibition map and clearly mark the exhibition areas that are closely related to your business or research field. For example, professionals engaged in molecular diagnostics should pay close attention to booths related to molecular diagnostic technology, which may showcase new gene sequencing equipment, PCR reagents, and cutting-edge molecular diagnostic technologies. After identifying key areas, targeted visits can avoid blindly wandering around the exhibition, save time and energy, and obtain valuable information for oneself. 2, Actively participate in forums and lectures to acquire cutting-edge knowledge The various forums, lectures, and seminars held during the exhibition are a gathering place for industry wisdom. The speakers of these activities are often experts, scholars, and entrepreneurs in the industry, who bring content covering new research findings, market trends, and policy interpretations. Get the schedule of forums and lectures in advance, and select relevant activities based on your interests and needs. Actively participating in these activities not only broadens one's horizons, but also enables interaction and communication with the speaker and other attendees. 3, In depth communication to gain professional insights from exhibitors Every exhibitor is a participant in the industry, with their own insights into market changes, technological developments, and customer needs. When communicating with them, boldly raise your own questions and concerns, whether it is about product performance, application scenarios, or industry development trends, you can obtain professional opinions and suggestions from them. 4, Observe competitors and gain insight into the market competition situation At the exhibition, the performance of competitors is an important window to observe the market competition situation. Pay attention to their product displays, promotional strategies, and customer interaction methods. From product displays, one can understand the product advantages and innovation points of competitors, compare their own company's products, and identify gaps and advantages; The promotional strategy can reflect their market positioning and promotion ideas; Customer interaction can help us understand customers' attention and feedback on competitor products. 5, Peer exchange, common progress The exhibition is a platform that gathers domestic and foreign IVD peers, taking advantage of this opportunity to exchange experiences and insights with peers from different regions. Colleagues have different practical experiences and innovative ideas in their respective fields. Through communication, they can learn from each other and broaden their horizons. 6, Collect data and samples to provide a basis for subsequent learning and analysis Don't miss the opportunity to collect various promotional materials, product samples, and technical documents during the exhibition. These materials are valuable gains from participating in the exhibition, as they provide detailed records of exhibitors' product information, technical parameters, and application cases. Sort and categorize these materials as a basis for subsequent learning and analysis. After the exhibition, one can delve into these materials to further understand the details of the products and technologies, providing reference for the company's procurement decisions, product development, and market expansion. The CACLP event in 2025 provides a platform for IVD professionals to learn, exchange, and collaborate. By clarifying key visiting areas, actively participating in forum lectures, engaging in in-depth exchanges with exhibitors and peers, and collecting data samples, one can gain rich knowledge and information at this grand event, bringing new opportunities for the development of their own enterprise. We look forward to every IVD person gaining a lot and reaching new heights in 2025CACLP.

Everyone has the love of beauty. When it comes to skin care, you have to mention aloe vera gel as the most versatile skin care product in the skin care industry. Aloe vera gel, the ingredients are extracted from a miraculous plant-aloe. Aloe vera juice contains a variety of nutrients and is widely used in daily life. However, the freshly extracted aloe vera has poor compatibility with the skin, fast volatilization and other characteristics, which are not suitable for direct application to the skin. It needs the assistance of another substance-carbomer. The two cannot be dissolved directly. Here is a preparation scheme of aloe vera gel and carbomer gel. We need to use triethanolamine plus a little antibacterial agent to make the final aloe vera gel product can be stored longer. Put the carbomer powder into an empty jar and stir quickly until it is uniform. If there is agglomeration, use a stir bar to break it. Or just add half the amount of water, and add the rest before the antibacterial agent after the transparent gel is formed . Set aside and stir occasionally until the carbomer fully absorbs water and swells and no lumps are visible. It takes about 2 to 3 hours for the gel to fully expand and form. If you want to shorten the gel formation time, you can use the water-proof heating method, and stir while heating. It takes about 10 to 30 minutes. Then add alkali to neutralize. Add slowly drop by drop, add and stir while measuring the pH value. At pH 7, it becomes a transparent gel. This step is very important and is the key to the success or failure of forming a transparent gel. When making the first time, it is best to add a drop of neutralizer-stir-measure the pH value, and repeat this step until the pH value reaches 7. This can be easily grasped When it comes to the preparation technology, the next step is very easy. Finally, add the antibacterial agent, stir evenly, pour into the extracted aloe vera gel, add glycerin, hyaluronic acid, polyglutamic acid and pure water to adjust the viscosity . In this way, the aloe vera gel is made. Aloe gel itself is a very pure pure plant ingredient extracted from aloe. However, most of the transparent gel-like ingredients on the market are not aloe vera gel, but are made of water-added gels (carbomer, etc.). This kind of jelly-like, very transparent gel substance is actually made by adding a lot of water and chemical moisturizing ingredients: (Carbomer). According to different usage scenarios, there are 100% and 92% different components to choose from. When buying aloe vera gel products, you must pay attention to the ingredients and choose the one that suits you. Desheng Biochemical is a high-tech enterprise focusing on the fields of life science and biotechnology. Based on the tenet of serving customers and serving scientific research, the company provides Chinese experts and scholars with a complete range of high-quality products. The carbomer, tris, Bicine, CAPS, MOPS, TAPS, HEPES, etc. produced have been widely used in life science basic research, development and application, biotechnology and many other fields.

The full name of TOOS reagent in Chinese is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, which is a chromogenic substrate reagent. Chromogenic substrates, also known as new Trinder's reagents witch are relatively common reagents in clinical biochemical testing projects, and TOOS is one of the most widely used chromogenic substrates. TOOS can be used in blood glucose monitoring, routine liver function detection, triglyceride and other blood lipid detection projects. The TOOS developed and produced by Desheng is a white powdery substance, which has obvious advantages compared with other manufacturers in terms of appearance and performance.Our TOOS has higher molar absorbance and more sensitivity than conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity, and the color of product is more stable.It not only shows good stability in some small measurement,the shape and color of the crystal powder are also purer, and the purity has reached 99%. Due to the special properties of TOOS, the prepared solution should be used at once. The solution will be oxidized and discolored when it exposed the air for a long time.This powdery substance is also more convenient to store, and its high solubility in water makes it easy to use even if it is configure before using. As a original manufacturer of TOOS, Desheng has been improving in terms of quality, price and service, adhering to the business philosophy of customer first. We are constantly making our services and products better for getting more affirmation from clients. As the saying goes,as long as you try very hard, your effort is rewarded.For 20 years harder working,Desheng has also successively gained the recognition and affirmation of more than 400 customers at home and abroad. We will continue to forge ahead.

TRIS is a commonly used biological buffer. It is widely used in the field of biochemistry and is also an important chemical raw material. For example, common Tris-HCL, TAE, TES, Bis-Tris, etc. are synthesized from it. . This article will introduce the TRIS derivative TES and Bis-Tris in detail. 1. TES (Tris ethanesulfonic acid) TES Chinese name: Tris ethanesulfonic acid, CAS number: 7365-44-8, TES has a structure similar to Trizma, and is one of the ethanesulfonic acid series physiological buffers developed by Good et al. It meets various standards of "Good" buffers , although the structure is a neutral molecule, it still behaves as a zwitterion in solution. TES has a pKa of 7.4 (physiological pH), making it a very versatile biological buffer. TES is suitable for use in a variety of cell culture systems that require divalent metal ions, whereas many other buffers (such as citrate or phosphate) cause chelation or precipitation reactions that make this requirement unsatisfactory. In addition, TES is extremely beneficial to study the succinic acid oxidation reaction. 2. Bis-Tris (bis-trimethane hydrochloride) Bis-Tris full name in Chinese: Bis (2-hydroxyhexyl) amino (trimethylol) methane, CAS number: 6976-37-0, is an inert zwitterionic buffer, its solution varies with temperature There are only slight changes from the constant. Bis-Tris can ionize hydrogen ions in a small amount, has weak acidity, and is suitable for weakly acidic buffer systems. Due to the introduction of two hydroxyethyl groups in Tris, there are many atoms providing lone pairs of electrons, and it has complexing ability for some metal ions. EDTA or Bicine are similar. Bis-Tris is suitable for the separation of hemoglobin, and can protect the stability of hemoglobin during freeze-dried storage. Useful as an effective buffer for assays of muscle enzyme activity. 2-D gel electrophoresis is used as the catholyte in isoelectric focusing electrophoresis (IEF). Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of raw materials for diagnostic reagents. It can provide a variety of biological buffers, including Tris base , Tris-HCl, Bis-Tris, Bicine, TAPS and other reagents. If you need to buy, please feel free to contact us !

Potential cross-contamination of additives between primary blood collection tubes is a common problem during sample collection, and contaminated additives have a significant impact on blood test results. Citrate blood contamination with varying amounts of dipotassium ethylenediaminetetraacetate (K2 EDTA blood) had significant effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrinogen.Fifteen samples containing healthy blood were collected into 0.109m vessels witch 4pcs with citrate and 1 pc with K2 EDTA. Combine four citrate tubes and divide into five equal portions. Then add whole blood sample with K2 EDTA in scalar amounts to autologous citrated blood aliquots to obtain 0% to 43% polluted K2 EDTA .Resulting statistically and clinically significant with prolongation of APTT and PT was observed between 29% and 43% K2 EDTA contamination,while fibrinogen values reduced at 43% K2 EDTA contamination.It indicates that contamination of citrated blood with up to 29% of K2 EDTA blood can cause significant bias in routine coagulation test results.This has serious implications for patient safety and management. Contamination of serum or lithium heparin blood with ethylenediaminetetraacetic acid (EDTA) salts may affect the accuracy of some key analytes and compromise patient safety.Combined 15 tubes containing lithium heparin and one tube of K2 EDTA and divided into 5 equal aliquots.Then add the whole blood from the K2EDTA tube in scalar amounts to the autologous heparinized aliquots to obtain varying degrees of contaminated K2 EDTA blood volume ( 0%; 5%; 13%; 29%; 43%). The following clinical chemistry parameters were then measured in centrifuged aliquots: alanine aminotransferase (ALT), bilirubin (total), calcium, chloride, creatinine, iron, lactate dehydrogenase (LD), lipase , magnesium, phosphate, potassium, sodium. Results show significant decreased in calcium, chloride, iron, LD, magnesium and increased potassium starting with 5% K2 EDTA contamination.Phosphate increased after 13% K2EDTA contamination while sodium increased after 29%. Values for ALT, bilirubin, creatinine and lipase remained unchanged until K2 EDTA contamination reached 43%.Compared changes to ideal quality specification,deviation were significant for calcium, chloride, LD, magnesium, potassium from 5% K2 EDTA contamination,and sodium, phosphate,iron from 29% K2 EDTA contamination. Concentrations of calcium, magnesium, potassium, chloride, and LD appear to be big biased even with 5% K2 EDTA contamination . Iron, phosphate, and sodium values remained reliable up to 29% K2EDTA contamination, while ALT, bilirubin, creatinine, and lipase appeared to be less susceptible to K2 EDTA contamination overall. Therefore, when saving blood collection tube additives, it is necessary to form a complete quality system according to the requirements of the corresponding product standards of the additives. In order to ensure that the additives can be used safely and effectively,the product’s MSDS should be prepared according to the requirements of GB/T16483.Material tolerance should be confirmed based on GB18280,then cobalt 60 irradiation sterilization followed.The blood collection tube additives produced by Desheng are produced and stored in strict accordance with the requirements of Pharmacopoeia and MSDS. 

3-(N-ethyl-m-toluidino)-2-hydroxypropane-sulfonic acid sodium salt (abbreviation is TOOS) is also named Sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate, which is a white powder with molecular formula of C12H19NO4S.Na and molecular weight is 331.36. TOOS is also called EHSPT, is often used in the following test kits: adenosine dehydrogenase detection kits for liver function testing, 5'-nucleotidase detection kits, glucose detection kits in blood glucose metabolism, glycation Albumin detection kit, 1,5-anhydroglucitol detection kit.TOOS with good characteristics water-solubility, high sensitivity and strong stability. The new Trinder's reagents are highly water-soluble aniline derivatives that are widely used in diagnostic assays and biochemical tests. They have several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity.The new Trinder's reagents are stable enough to be used with both in solution and experimental pipeline detection systems. With hydrogen peroxide and peroxidase, the new Trinder's reagent react with 4-aminoantipyrine (4-AA) or 3-Methylbenzothiazolesulfonehydrazone,and form a very stable purple or blue dye.The molar absorbance of dye formed by new trinders’ reagent reacting with MBTH is 1.5-2 times higher than with 4-AA; But MBTH solution is more stable. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of this hydrogen peroxide corresponds to the concentration of the substrate. Therefore, the amount of the substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol , acyl-CoA, and cholesterol can be used to detect those substrates coupled to the Trinder's reagents and 4-AA.TOOS is the most commonly used among 10 novel trinder’s reagent.However, it is necessary to develop more kinds of trinder’s reagent to match the specific substrates.     The chromogenic reagents produced by Desheng are impeccable in terms of purity, sensitivity, stability and appearance. The strict control of raw materials by quality inspection department from storage to production,guarantee the quality of chromogenic reagent.Only an enterprise that focuses on product research and development can provide customers with assurance. pls contact visit our website.

MOPS (3-(N-morpholino)propanesulfonic acid) and MOPSO buffer (3-(N-morpholinyl)-2-hydroxypropanesulfonic acid) in Good’s buffer It is two kinds of buffers that are widely used. From the English abbreviation, they only differ by one letter, so it is easy for the unfamiliar to confuse the two. In fact, there are both differences and similarities between MOPS and MOPSO. Next, I will briefly introduce their differences in application and configuration, as well as the common advantages of these two products. Differences between MOPS and MOPSO applications MOPS can be used in the following experiments: (1) As a running buffer in electrophoresis and for protein purification in chromatography; (2) It is formulated into a variety of agar media for the cultivation of bacteria, yeast and mammalian cells. However, concentrations higher than 20mM are not recommended for mammalian cells; (3) It can be used as a lysis buffer for E. coli cells; (4) as an eluent in gel filtration chromatography; (5) Applied to Northern hybridization as a buffer for RNA isolation and membrane transfer; (6) It is suitable for the determination of bisquinolinecarboxylic acid (BCA); (7) Electron transport and phosphorylation studies for chloroplast thin layer preparation. MOPSO can be used in the following experiments: (1) Used as a carrier electrolyte in capillary electrophoresis, and a crystallization buffer for glutathione synthase; (2) Used in fluorescence spectroscopy, spectrophotometry and isothermal titration calorimetry; (3) Interact with the peptide backbone of bovine serum albumin (BSA) to prevent thermal denaturation of BSA; (4) As one of the buffer components for copper-related analysis; (5) It is used as a component of buffered charcoal yeast extract medium; (6) Prepare MOPSO-ethanol buffer system to fix urine-derived cells; (7) It is used as a buffer system for the determination of by-products of biological treatment of marine crude oil. The difference between MOPS and MOPSO configuration How to configure MOPSO 1. Prepare 800 mL of distilled water (dH2O) in a beaker; 2. Put 112.63 grams of MOPSO powder into a beaker filled with distilled water (dH2O); 3. Adjust the solution in the beaker to the desired pH value with (10N NaOH); 4. Add distilled water (dH2O) until the volume is 1L and the configuration is complete. How to configure MOPS 1. Weigh 41.8g MOPS and place it in a 1L beaker; 2. Add about 700ml of DEPC treated water, stir to dissolve; 3. Use 2N NaOH to adjust the pH to 7.0, then add 20 ml of 1M NaOAc (DEPC treatment) and 20 ml of 0.5M EDTA (pH 8.0, DEPC treatment) to the solution; 4. Dilute the solution to 1L with DEPC-treated water; Filter with a 0.45um filter to remove impurities, and store at room temperature in the dark. Common advantages of Desheng MOPSO and MOPS: 1. The purity of Desheng MOPSO and MOPS both reach ≥99%, good water solubility, stable process, and can control a constant pH range for a long time. A full range of product testing and special testing services can be provided according to your application needs. 2. Desheng has a professional R&D and production team with a daily output of 1-2 tons and advanced production technology and equipment. There will be no long supply cycle or supply interruption. 3. For customers, we enjoy the advantage of trial packs. 4. The conventional packaging is 20KG/drum, but as a manufacturer, we can pack according to the needs of customers. 5. With strong logistics capabilities and rich export experience, we have exported to more than 100 countries and have established long-term cooperation 

In order to resist the influence of a small amount of strong acid and based on biochemical research work, we often use an important reagent-buffer solution. Tris(hydroxymethyl)aminomethane, CAS corresponding number 77-86-1, is an important member of the buffer family, and is also widely used in the preparation of buffers in biochemistry and molecular biology experiments. It is also used for the preparation of surfactants and vulcanization accelerators; and the preparation of water-soluble polymers using Tris structural units as coating dispersants. The advantages of TRIS buffers are also obvious. Because Tris base has a certain alkalinity, it can be used to prepare buffers with a wide range of pH from acidic to alkaline. Tris has little interference to the biochemical process and will not precipitate with calcium, non-heavy metal magnesium and heavy metal ions. There are two ways to prepare Tri-HCl buffer: use 0.05 mol/L Tris and 0.05 mol/L HCl solution, and then mix according to the volume listed in the common table. However, the standard concentration of dilute hydrochloric acid is not easy to obtain, so another method is usually used: Take 1 liter of 0.1 mol/L Tri-HCl buffer solution as an example: first, use 12.11 grams of Tris base to dissolve it in 950 mL~970 mL of deionized water, then add 4 N HCl while stirring. Use a pH meter to measure the pH of the solution to the desired pH, and then add 1L of water. Tris buffer is a buffer widely used in biochemical research. The common effective pH range is in the "neutral" range, for example: Tris-HCl buffer: pH = 7.5~8.5 Tris-phosphate buffer: pH = 5.0~9.0 In the electrophoresis buffer solution, glycine can form a stable pH Value buffer system. The Tris-HCl buffer system is used to stabilize the pH of the gel. It is also widely used as a solvent for nucleic acids and proteins. The synthesis of nematode intermediate fibers also uses the low ionic strength of Tris buffer. Add EDTA to Tris hydrochloric acid buffer to prepare TE buffer. This buffer can be used for DNA structure stabilization research and storage. The "TAE buffer" is obtained by substituting acetic acid for the pH adjusting acid solution, and the TBE buffer is obtained by substituting boric acid. Two commonly used buffers are used for nucleic acid electrophoresis experiments. Desheng Biochemical is a high-tech enterprise specializing in biotechnology and life fields. The company serves customers and takes scientific research services as its purpose. Its high-quality products have been adopted by scholars and experts from all over the world. The production of TRIS, TRIS-HCl, BICINE/CAPS/MOPS, Taps/hepes and other products has been widely used in basic research, epidemic diagnosis, control, and basic research in the development or application of many fields such as biotechnology. Customers are all over universities, frozen commodity quarantine and inspection, pharmaceutical production companies, biotechnology companies, food industry and other departments.

Desheng company is established for 20 years, focusing on R & D, production and sales in one of the old company, R & D and production of a complete range of products, price concessions, now on our company's R & D and production experience α- Glucosidase is a simple explanation. Glucosidase is one of the major enzymes in glycoside hydrolases (EC 3.2.1). It is named because it can hydrolyze glucoside bond and release a molecule of glucose. Glucosidase is one of the important members of glucose metabolism pathway. β- Glucosidase is involved in the metabolism of cellulose and many physiological and biochemical pathways, α- Glucosidase is directly involved in the metabolism of starch and glycogen. It has double functions of hydrolysis and transglycoside in the catalytic reaction of sugar. Hydrolysis can be carried out from α- Non reducing telostomy of glucosides, oligosaccharides and glucans α- 1,4 glycosidic bond, releasing glucose; In addition, the free glucose residues can be separated by transglycosylation α- Non fermentative Isomaltooligosaccharides (IMO) were obtained by transferring 1,6 glycosidic bond to another glucose or maltose substrate. The abnormal function of these enzymes will lead to metabolic diseases. At the same time, these enzymes are also the targets of many drugs and inhibitors to regulate the glycochemical metabolism in the human body. α- Glucosidase can be used to screen active natural drugs; one α- Immobilization of glucosidase: using trimethylol phosphorus as crosslinking agent and chitosan as carrier α- Glucosidase; 2. Make inhibitor screening model: immobilize the above-mentioned inhibitors α- The glucosidase was loaded into a column with a diameter of 0.8 cm and a length of 8 cm, in which the pH 6.8 potassium phosphate buffer was added and stored at 4 ℃; 3. Validation of the model: a representative α- Acarbose, a glucosidase inhibitor, was used to verify the effectiveness of the screening model. The lower end of the column was tightly inserted into a small tube with a piston, and a syringe was inserted into the upper end. The buffer solution in the column was pressed to about 0.4cm below the top of the column, and then 50 μ L 4-nitrobenzene- α- D-glucopyranoside (0.116mol / L) and a certain amount of acarbose solution (250mg / L), gently shake to make the solution at the top of the column mix well, then continue to press the solution until the liquid level is level with the top of the column, close the bottom piston, then put the column into the water solution tank, incubate at 37 ℃ for 10 minutes, after the reaction is completed, use 5ml Wash the column with ph6.8 potassium phosphate buffer, collect the washing solution, dilute the eluent to 10ml volume, and determine the absorbance value at 400nm with ultraviolet spectrophotometer; And the above 50 μ L 4-nitrobenzene- α- D-glucopyranoside, acarbose solution and 5ml buffer solution were diluted to 10ml as blank, and the inhibition rate of acarbose was calculated according to the absorbance data of the obtained reaction solution; 4. Immobilization α- Screening model of glucosidase for water soluble fraction of traditional Chinese medicine Polygonum cuspidatum α- Objective to screen the water-soluble fraction of Polygonum cuspidatum. We Desheng R & D and production of enzyme preparations used in the kit, customers with relevant needs are welcome to consult.

Carbomer is also known as acrylic resin, polyacrylic acid, carbomer resin, carbopol or carbopol. The appearance is white loose powder with a slight special smell. This product is very easy to absorb moisture, so it is placed in a ventilated The warehouse is protected from light and dry to ensure product quality. Carbomer 940 uses: short rheology, high viscosity, high clarity, low ion resistance and shear resistance, suitable for skin care lotions, creams, alcohol-containing transparent gel products, transparent skin care gels, hair Use styling gel, traveling wave, shower gel, recommended dosage: 0.2-1.0%. Carbo 940 has short rheological properties, and the viscosity reaches 63000MPA.S at 0.5%, which is suitable for products with high viscosity. Precautions for use of Carbomer 940: Long-term stirring or high-shear stirring after carbomer neutralization will cause viscosity loss;The presence of electrolyte will reduce the thickening efficiency of carbo resin;Long-term ultraviolet radiation will reduce the viscosity of carbomer. Finally, Carbomer is soluble in water, glycerol and ethanol, and has the characteristics of colloidal solution. The usual concentration is 0.1% ～ 3%. Because its molecular structure contains 52% ～ 68% acidic groups, it has certain acidity. The pH value of 1% water dispersions is 2.5 to 3, and the viscosity is relatively low. When neutralizing with alkali, the viscosity gradually increases with the gradual dissolution of macromolecules, forming a clear solution at low concentration, forming a semitransparent gel at a high concentration. The gel has the greatest viscosity and consistency at pH6 ~ 12, and the viscosity decreases when pH < 3 or pH > 12. Commonly used neutralizers include triethanolamine, ethylenediamine, laurylamine, sodium bicarbonate, sodium hydroxide, etc. generally, 1 g carbomer needs 1.35g triethanolamine or 400mg sodium hydroxide to neutralize. Strong electrolytes and sunlight can reduce the viscosity of the gel. The temperature also has some effect on the viscosity, but the relative change is not great. The gel is best preserved in the refrigerator.