Guidelines for the use of EDTA dipotassium 25102-12-9 anticoagulant tubes: key points to avoid hemolysis and sample errors

In modern medical diagnosis, the accuracy of blood samples is crucial. EDTA (ethylenediaminetetraacetic acid) dipotassium anticoagulant tube is a commonly used blood collection tool, and its correct use directly affects the quality of the sample and the reliability of the diagnostic results. Below, we will specifically discuss the precautions to be taken when using EDTA dipotassium anticoagulant to avoid hemolysis and sample errors.

Preparation and addition of anticoagulant solution

1. Preparation of anticoagulant solution. The preparation of EDTA dipotassium anticoagulant is a crucial step before blood collection. The EDTA dipotassium salt should be dissolved in deionized water to ensure that the solution does not contain impurities that may affect the anticoagulant effect. During the dissolution process, it is necessary to stir thoroughly or heat at an appropriate temperature to accelerate the dissolution. However, attention should be paid to temperature control to avoid the decomposition of EDTA dipotassium salt, which may affect anticoagulant performance. At the same time, accurately weigh the EDTA dipotassium salt to ensure that the solution concentration meets anticoagulant requirements.

EDTA dipotassium powder

2. Addition of anticoagulant. The amount of anticoagulant added should be strictly controlled, whether it is machine added or manually added, to ensure the accurate addition of EDTA dipotassium. When adding the machine, the container and conveying pipeline should be cleaned and disinfected to avoid contamination. The exact amount added should be determined based on the volume of the blood collection tube, and the machine parameters should be adjusted to ensure that the amount added meets the industry standard dosage range; When manually adding, a calibrated pipette should be used and operated strictly according to the operating procedures to ensure accurate addition.

Blood collection and mixing

When collecting blood samples, follow the principle of aseptic operation to avoid contamination. After collecting blood, it is necessary to gently invert the test tube several times to ensure that the blood is fully mixed with EDTA dipotassium anticoagulant. Gently shake or flip the test tube to evenly distribute the anticoagulant and avoid violent shaking that may cause hemolysis. Hemolysis can release hemoglobin, interfere with biochemical indicators testing, affect the red blood cell count results in blood routine testing, and even interfere with coagulation function testing.

Preventive measures for hemolysis and sample error

1. Avoid hemolysis. Hemolysis is one of the main factors affecting the quality of blood samples. During the collection, mixing, storage, and transportation processes, the operating procedures should be strictly followed to avoid factors that may cause hemolysis, such as shaking the test tube with force, high or low temperature, contamination, etc. At the same time, regularly check the quality of blood collection equipment and anticoagulant tubes to ensure that they are intact and undamaged.

2. Control the ratio of EDTA dipotassium anticoagulant. The ratio of EDTA dipotassium anticoagulant to blood is a key factor affecting sample quality. Excessive use of EDTA dipotassium anticoagulant can lead to blood dilution effects, affecting cell counting and biochemical parameter measurements; Insufficient EDTA dipotassium anticoagulant prevents effective anticoagulation, leading to sample coagulation. Therefore, the dosage of anticoagulants should be strictly controlled to ensure that their ratio to blood meets industry standards.

3. Regularly calibrate equipment. Regularly calibrate blood analysis equipment to ensure its accuracy and stability. Use precision pipettes and automatic separators, strictly follow standard operating procedures to avoid detection errors caused by equipment failure or inaccurate calibration.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. has been researching and producing blood collection tube additives such as EDTA dipotassium for nearly 20 years. The performance of various products produced not only meets relevant standards, but also meets the customized needs of customers. If you are interested in our products, please click on the official website to learn more details or contact me!