What details should be paid attention to during the labeling process of acridine ester NSP-SA-NHS 199293-83-9

The application of chemiluminescence reagents is becoming increasingly widespread in fields such as biomedicine, environmental monitoring, and drug analysis. Among them, acridine ester NSP-SA-NHS is highly favored as an efficient chemiluminescence marker due to its good stability, activity, and sensitivity. However, when using acridine ester NSP-SA-NHS for labeling, a series of details need to be strictly followed to ensure the accuracy of experimental results and the stability of reagents. This article will explore these precautions in detail to assist researchers in successfully completing experiments.

Storage and preparation of reagents

1. Seal and avoid light

Acridine ester NSP-SA-NHS is extremely sensitive to light, which can cause partial decomposition and affect the luminescence effect. Therefore, it is necessary to strictly avoid light during the experimental process and product storage. It is recommended to store the reagents in well sealed, opaque dark bottles and keep them in a cool place.

acridine ester NSP-SA-NHS powder

2. Low temperature storage

To prevent the reagent from absorbing moisture and deteriorating, the acridine ester NSP-SA-NHS needs to be stored at low temperatures. It is usually recommended to store the reagents in the refrigerator and regularly check the sealing to ensure that the reagents are not damp. When using, it should be taken out in advance according to experimental needs to avoid condensation of water vapor caused by temperature differences.

3. Solvent selection

Before labeling, due to the activation of the terminal carboxyl group of acridine ester NSP-SA-NHS by NHS, which is more active, non protonic dry anhydrous solvents such as anhydrous DMSO or DMF should be used for dissolution. Ensure the quality and purity of the solvent to avoid interference with the labeling process.

Optimization of Marking Conditions

1. Preparation of marking buffer solution

The preparation of labeling buffer is crucial for the labeling effect. Usually, it is recommended to use 0.2M NaHCO3 (pH=9.0) as the labeling buffer to provide a suitable alkaline environment. In addition, other suitable buffer solutions can be selected according to experimental requirements, such as 20mM PBS.

2. Control of marking ratio and time

During the labeling process, the molar ratio of antibodies (proteins) to acridine esters needs to be adjusted according to experimental requirements. In addition, the length of the marking time will directly affect the marking effect.

Purification and detection of labeled products

1. Purification of labeled products

After labeling, the labeled product needs to be purified by dialysis, desalination column, or ultrafiltration to remove unreacted acridine esters and other impurities. For large molecule compounds such as proteins, it is recommended to use a desalination column for separation; For small molecule compounds, it is recommended to use column chromatography for separation.

2. Detection of marking efficiency

The efficiency of detecting acridine ester labeling mainly depends on its chemiluminescence properties. By measuring the luminescence intensity exhibited by labeled biomolecules in alkaline hydrogen peroxide solution, the efficiency of labeling can be indirectly reflected. The usual practice is to compare the luminescence intensity data obtained under different labeling conditions (such as different concentrations or reaction times) to determine the optimal labeling conditions.

Product packaging

In addition to the acridine ester NSP-SA-NHS, the chemiluminescence reagents produced by Desheng also include products such as luminol, isoluminol, and luminol monosodium salt. The product quality is high, the production process is stable, and the batch differences are small. There is a large amount of stock available and the delivery speed is fast. If you need it, please contact us now!