When Using Luminol 521-31-3 To Detect Bloodstains , These Details Must Be Remembered Carefully

Luminol plays a crucial role as an efficient chemiluminescence reagent in criminal investigation, forensic identification, and various situations that require precise identification of bloodstains. It can react with hemoglobin in the blood and emit strong blue light under appropriate conditions, revealing trace bloodstains that are difficult to detect with the naked eye. However, in order to fully utilize the detection efficiency of luminol and ensure the accuracy and reliability of the results, a series of operational details must be strictly followed.

1, Careful preparation during the preparation phase

1.1 Environmental Control

Light conditions: Luminol luminescence reaction is sensitive to light, and detection should be carried out in a dark room or low light environment to reduce external light interference and improve detection sensitivity.

luminol reagent powder

Temperature and humidity management: Maintain appropriate temperature (usually between 15-25 ° C) and humidity (avoid extreme humidity or dryness), as these factors may affect the reaction efficiency of luminol.

1.2 Preparation of reagents

Purity and storage: Ensure that the luminol reagent used is of high purity to avoid impurities interfering with the reaction. When storing, it should be kept away from heat sources and direct sunlight, and kept sealed and dry.

Solution preparation: Accurately prepare the luminol solution according to the instructions, pay attention to the selection and proportion of solvents, and adjust the pH value of the solution, which will affect the luminescence effect.

2, Rigorous execution during the operation process

2.1 Preprocessed Samples

Clean the surface: Before spraying luminol, thoroughly clean the surface to be inspected to remove any stains or grease that may affect the reaction.

Drying treatment: Ensure that the sample is completely dry, as moisture can interfere with the contact between luminol and hemoglobin, affecting the luminescence reaction.

2.2 Uniform spraying

Spray technique: spray Luminol solution evenly with a fine spray nozzle to avoid excessive solution flowing and affecting the detection accuracy.

Comprehensive coverage: Ensure that the inspection area is fully covered, especially the edges and hidden corners, to avoid missing important clues.

2.3 Observation and Recording

Observation timing: Start observing immediately after spraying, usually the luminescent reaction can be manifested within a few seconds, and the duration is relatively short.

Detailed recording: Use a professional camera or equipment suitable for low light environments to record the illuminated images, while annotating information such as time and location for subsequent analysis.

3, Scientific interpretation of results

3.1 Interpretation of Luminous Intensity

The relationship between intensity and blood volume: The luminescence intensity is usually proportional to the amount of blood, but it should be noted that the luminescence of old or contaminated blood may be weakened.

Identification of interference factors: Certain chemical substances (such as bleach, rust) can also cause luminescence of luminol, which needs to be comprehensively judged based on the on-site situation.

3.2 Verification and Review of Results

Multi angle observation: Take multi angle photos of suspected bloodstains to confirm the three-dimensional distribution of luminescence and reduce misjudgment.

Re examination mechanism: For key evidence, it is recommended to use other testing methods (such as DNA analysis) for re examination to ensure the accuracy and authority of the results.

luminol reagent

Hubei Xindesheng Material Technology Co., Ltd., as a high-quality manufacturer of chemiluminescence reagents, has rich experience in research and development and production. After customers purchase products, we will continue to track and guide them to use the products correctly! If you have any purchasing needs in the near future, please click on the official website of Desheng to learn more details!