Is Biological Buffering HEPPSO 68399-78-0 Corrosive?

In biochemical experiments, buffering agents play a crucial role in maintaining the pH stability of solutions, ensuring the accuracy and reliability of experimental results. 3- (Hydroxyethylpiperazine) -2-hydroxypropanesulfonic acid (HEPPSO), as an important zwitterionic buffer, has attracted much attention due to its unique chemical properties and wide range of applications. This article mainly discusses whether HEPPSO has corrosiveness from three aspects: chemical properties, corrosiveness assessment, and application.

Basic properties of HEPPSO

HEPPSO, The chemical formula is C9H20N2O5S, with a molecular weight of 268.33g/mol, and it is a white crystalline powder. It is soluble in water, methanol, ethanol, and most organic solvents, demonstrating good solubility. The melting point of HEPPSO is 158-160 ° C, the predicted density is 1.382 ± 0.06 g/cm3, the pKa value is 7.84, and the pH range is 7.1-8.5. These physical and chemical parameters indicate that HEPPSO is a stable compound that can be stored for a long time without decomposition, but it should be noted that it is sensitive to moisture and should be stored in a dry environment.

HEPPSO powder

Corrosion assessment of HEPPSO

Regarding the issue of whether HEPPSO is corrosive, we can consider the following aspects:

1. Chemical properties: From a chemical perspective, HEPPSO is a zwitterionic buffering agent, which contains functional groups such as hydroxyl and sulfonic acid groups in its molecular structure. These functional groups can form hydrogen bonds with water molecules in solution, thereby playing a buffering role. However, these functional groups do not possess strong oxidizing or reducing properties, so HEPPSO itself is not corrosive.

2. Data verification: In practical applications, many researchers and experimenters have verified the safety of HEPPSO through a large amount of experimental data. Under normal usage and storage conditions, HEPPSO will not cause corrosion or injury to experimental equipment, biological samples, or operators.

3. Precautions for use: Although HEPPSO itself is not corrosive, there are still some precautions to be taken during use. For example, HEPPSO is sensitive to moisture and should be stored in a dry environment; When preparing solutions, the correct proportions and methods should be followed; During use, contact with corrosive substances such as strong acids and alkalis should be avoided.

The purpose of HEPPSO

1. Maintaining pH stability of the solution: The main function of HEPPSO is to maintain the pH stability of the solution. In processes such as cell culture, protein separation, and purification, a stable pH environment can support cell growth and maintain physiological activity, protecting the biological activity of proteins.

2. Cell culture: As a buffer in the culture medium, HEPPSO can provide a suitable pH environment, promote cell growth and division, and improve the success rate of cell culture.

3. Protein separation and purification: HEPPSO acts as a stabilizer in the process of protein separation and purification, which can protect the biological activity of proteins and improve separation efficiency.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality supplier specializing in the production of biological buffering agents, with nearly 20 years of research and development and production history so far. The purity of HEPPSO and other biological buffering agents produced by Desheng Company can reach over 99%, with good solubility and stable chemical properties, which are highly welcomed by users. If you have any recent purchasing needs, please click on the official website to learn more details or contact me directly!