The Key Role Of MOPSO 68399-77-9 In Glycated Albumin Assay Kit

In the field of biochemistry and medical diagnosis, the determination of glycosylated albumin is a crucial experiment, which is of great significance for the diagnosis and treatment of metabolic diseases such as diabetes. In this assay, the biological buffer 3- (N-morpholino) -2-hydroxypropanesulfonic acid (MOPSO buffer) plays a crucial role.

Basic characteristics of MOPSO buffer solution

MOPSO, The full name is 3- (N-morpholino) -2-hydroxypropanesulfonic acid, which is a zwitterionic buffer belonging to the Good's buffer series. It is usually in the form of white crystalline powder, with good water solubility, and is not easily affected by factors such as temperature. These characteristics make MOPSO an ideal biological buffer, widely used in various biochemical research and diagnostic kits.

MOPSO powder

MOPSO exhibits excellent buffering ability over a wide pH range, and the morpholine and sulfonic acid groups in its molecular structure endow it with unique physicochemical properties, including high solubility, low toxicity, and good biocompatibility. Of particular importance is the molecular size, shape, and charge distribution characteristics of MOPSO, which make it difficult to penetrate biofilms composed of phospholipid bilayers. This characteristic is of great significance in biological research.

The role of MOPSO buffer in the determination of glycated albumin

The glycated albumin assay kit usually includes R1 reagent and R2 reagent, where R1 reagent is mainly used for the decomposition of glycated albumin, while R2 reagent is used for color reaction and final concentration determination. MOPSO plays an important role in both of these reagents.

1. Stable reaction environment

The determination of glycated albumin requires specific pH conditions. MOPSO, as a buffering agent, can provide a stable and suitable pH environment, ensuring smooth enzymatic and colorimetric reactions. Its pH buffer range precisely covers the conditions required for measuring glycated albumin, avoiding measurement errors caused by pH fluctuations.

In R1 reagent, MOPSO buffer provides a suitable pH environment for protease, ensuring the stable progress of glycated albumin decomposition reaction. At the same time, the ionic properties of MOPSO promote the interaction between protease and glycosylated albumin, improving the decomposition efficiency.

In R2 reagent, MOPSO buffer continues to play its role in stabilizing pH, ensuring the smooth progress of color reaction. At the same time, MOPSO also promotes the interaction between enzymes and substrates through its zwitterionic properties, improving the sensitivity and accuracy of color reactions.

2. Reduce interference factors

In the process of measuring glycated albumin, interference from metal ions and other chemical substances can have a negative impact on the results. MOPSO buffer not only does not precipitate with metal ions, but also effectively reduces the interference of other chemical substances, ensuring the accuracy and reliability of the measurement results.

3. Improve reaction efficiency

MOPSO buffer not only provides a suitable pH environment, but also promotes enzymatic reactions through its zwitterionic properties. In the determination of glycated albumin, MOPSO can optimize the activity of proteases, improve the decomposition efficiency of glycated albumin, thereby shortening the reaction time and improving the determination efficiency.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. has been developing and producing MOPSO and other biological buffering agents for nearly 20 years, with stable production processes and small batch differences in products. Each batch of goods sold will be accompanied by a quality analysis report issued by Desheng's quality inspection team to ensure that all indicators of MOPSO comply with relevant standards. If you have any recent purchasing needs, please click on the official website of Desheng to learn more details or contact me!