Unique Advantages Of Biological Buffer MOPSO In Fluorescence Spectroscopy

Basic properties of MOPSO

MOPSO, with the chemical formula C7H15NO5S and a molecular weight of 225.27, is a white powdery compound. Its pKa value is 6.95, suitable for solution environments with a pH range of 6.2-7.6. MOPSO, due to its unique chemical structure, not only exhibits good buffering performance, but also has high chemical stability and does not undergo chemical reactions with most metal ions, enzyme preparations, etc.

MOPSO buffer

The unique advantages of MOPSO in fluorescence spectroscopy

1. Stabilize pH value and optimize experimental environment

Stable pH range: MOPSO can maintain the stability of solution pH within a wide pH range, especially in the neutral to alkaline region. This is crucial for fluorescent molecules, as their emission characteristics, such as fluorescence intensity and spectral shape, are highly sensitive to pH.

Reducing spectral drift: By maintaining a stable pH environment, MOPSO reduces fluorescence quenching or spectral drift caused by pH fluctuations, thereby enhancing the stability and reliability of fluorescence signals.

2. Improve the quality of fluorescence signals

Reduce fluorescence quenching: MOPSO helps to reduce non-specific interactions between fluorescent molecules and other molecules by maintaining the stability of the solution environment, thereby reducing the resulting fluorescence quenching phenomenon. This is a common interference factor in fluorescence spectroscopy analysis, and the addition of MOPSO can significantly enhance the intensity and quality of fluorescence signals.

Protecting fluorescent probes: Photobleaching is a common issue during prolonged fluorescence observation or repeated measurements, which can cause the fluorescence signal to gradually weaken. MOPSO helps to slow down the photochemical degradation of fluorescent molecules, protect fluorescent probes, and prolong their effective luminescence time by providing a mild and stable environment.

3. Improve experimental accuracy and sensitivity

Chemical stability: MOPSO is inert to various chemical reagents and enzymes, and does not form complexes with most metal ions. This enables it to effectively function in fluorescent labeling systems containing metal ions or complex components, without interfering with the fluorescence properties of target molecules or causing adverse reactions.

Improving data reproducibility: The MOPSO produced by Desheng has small inter batch differences, which means that it can maintain a high degree of consistency in experimental conditions when used in multiple experiments or different laboratories. This is crucial for ensuring the comparability and repeatability of fluorescence spectral data, and is the foundation for quantitative analysis and scientific research.

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