Precautions For Using Chemiluminescence Reagent Acridine Salt NSP-SA-NHS 199293-83-9

1, Packaging and storage conditions

Sealed and dark proof: Acridine ester NSP-SA-NHS must be stored in a well sealed, opaque dark bottle. Due to the sensitivity of the reagent to light, light exposure can cause partial decomposition, thereby affecting the luminescence effect. Therefore, it is necessary to strictly avoid light during the experimental process and product storage.

Acridine ester NSP-SA-NHS powder

Low temperature storage: Acridine ester NSP-SA-NHS needs to be stored at low temperatures to prevent moisture absorption and deterioration. It is recommended to store the reagents in the refrigerator and regularly check the sealing to ensure that the reagents are not damp.

2, Dissolution and labeling

Solvent selection: Acridine ester NSP-SA-NHS can be dissolved in non protonic solvents such as anhydrous DMSO. Before labeling, due to the activation of the carboxyl group at the end of the acridine ester by NHS, it is more active and should be dissolved using non protonic dry anhydrous solvents.

Marking process: After marking, acridine ester exists in the form of amide or ester bonds with the labeled substance, and acidic solvents have little effect on the labeled substance. However, due to the instability of the active sites of acridine esters in alkaline and oxidative environments, corresponding non strong alkaline and non oxidative systems should be prepared based on the stability of the labeled substance for processing and preservation.

3, Experiment and Testing

The luminescence process is brief: The luminescence process of acridine ester NSP-SA-NHS is very brief, with the entire process occurring in less than 2 seconds. Therefore, when triggering the luminescence condition, it is necessary to prepare detection instruments in advance, such as internal photometers and photon detectors, to capture key information and ensure the accuracy of experimental and detection results.

Separation and purification: When labeling large molecular compounds such as proteins, it is recommended to use a G25 desalination column for separation; When labeling small molecule compounds, it is recommended to use column chromatography for separation. These separation methods can effectively remove unlabeled reagents and impurities, and improve the purity of labeled substances.

4, Stability and safety

Stability impact: The stability of acridine ester NSP-SA-NHS is greatly affected by temperature, pH value, and oxidation environment. The higher the temperature, pH value, and oxidation environment, the higher the degree of hydrolysis of the reagent, and the lower its stability. Therefore, during storage and experimentation, these conditions should be strictly controlled to extend the service life of the reagents.

Safety precautions: Although the toxicity of acridine ester NSP-SA-NHS is relatively low, safety precautions should still be taken during use. It is recommended to wear personal protective equipment such as protective goggles, gloves, and masks to avoid direct contact of reagents with the skin and eyes. At the same time, good ventilation conditions should be maintained during the experiment to prevent the volatilization of reagents from causing harm to the human body.

Product packaging