The Labeling Principle And Application Of Acridine Hydrazide NSP-SA-ADH

1, Understand the basic characteristics of acridine hydrazide NSP-SA-ADH

Acrylamide hydrazide NSP-SA-ADH is a fluorescent label based on the acridine structure, which appears as a yellow powder with a purity of over 98% and a molecular weight of 740.85. The specific active groups in its molecular structure can undergo chemical reactions with aldehyde groups in biomolecules, forming stable covalent bonds and achieving specific labeling of target molecules. This reagent not only has excellent fluorescence performance, but also has good stability and water solubility, making it easy to use under various experimental conditions.

acridine hydrazide NSP-SA-AH powder

2, Optimization of storage conditions

The correct storage conditions are the foundation for ensuring the stability of acridine hydrazide NSP-SA-AH. Due to its sensitivity to temperature and light, the reagent needs to be stored in a constant and suitable temperature environment. It is recommended to equip professional refrigeration or freezing equipment, control the temperature between 2-8 ℃, and regularly check the operating status of the equipment to ensure that temperature fluctuations are controlled within a small range. In addition, packaging bags or containers made of multi-layer composite materials should be selected to provide a good physical barrier to prevent direct effects of external temperature and humidity changes on the reagents. At the same time, these materials should also have good sealing and chemical corrosion resistance to ensure that the reagents remain stable during long-term storage.

3, Preparation before use

Before using acridine hydrazide NSP-SA-ADH, sufficient preparation work needs to be done. Firstly, it is necessary to carefully read and strictly follow the various regulations in the product manual, including the expiration date after bottle opening, dilution ratio, mixing sequence, etc. Secondly, as the reagent is stored under refrigeration, it needs to be taken out in advance and left for a period of time to adapt to room temperature before use, in order to avoid condensation of water vapor due to temperature differences, which may cause powder adhesion and make weighing inconvenient. In addition, due to the sensitivity of acridine hydrazide NSP-SA-ADH to light, its use should be carried out under light avoidance conditions as much as possible to reduce light induced degradation reactions.

4, Correct usage method

The correct usage method is the key to ensuring experimental accuracy and reliability. When using acridine hydrazide NSP-SA-ADH, the container containing the dissolution reagent can be directly peeled and weighed, and then dissolved in anhydrous DMF to avoid weighing loss or transfer process loss. Accurate electronic scales should be used for weighing, and the weighing environment should be kept clean and dry. During the dissolution process, the type and proportion of solvent, as well as the temperature and time of dissolution, should be strictly controlled to ensure sufficient dissolution and stability of the reagent.

5, Operating standards and precautions

When using acridine hydrazide NSP-SA-ADH, it is necessary to strictly follow the operating procedures to reduce reagent waste and performance degradation caused by improper operation. Firstly, professional training should be provided to researchers and operators who use reagents to understand their properties, storage requirements, and operating procedures. Secondly, during use, the performance of the reagents should be regularly monitored. Once signs of decreased stability are found, immediate measures should be taken to adjust or replace them to avoid adverse effects on the experimental results. In addition, attention should be paid to the cleanliness and sterility of the experimental environment to avoid contamination and cross infection.

Product packaging