Product Details
Place of Origin: EZHOU, CHINA
Brand Name: DESHENG
Certification: ISO9001:2008
Model Number: Chemiluminescent Reagent
Payment & Shipping Terms
Minimum Order Quantity: 10g
Price: Negotiable
Packaging Details: Plastic bottle/Ctn Box
Delivery Time: 1-3 days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100kg/Month
Name: |
Luminol Reagent |
Full Name: |
3-aminophthalic Hydrazide |
Appearance: |
Yellow Powder |
Molecular Formula: |
C8H7N3O2 |
Molecular Weight: |
177.16 |
Purity: |
＞98% |
CAS NO#: |
521-31-3 |
Category: |
Trinder's Reagent |
Application: |
Chemiluminescence Immunoassay |
Industry: |
Bioscience |
Name: |
Luminol Reagent |
Full Name: |
3-aminophthalic Hydrazide |
Appearance: |
Yellow Powder |
Molecular Formula: |
C8H7N3O2 |
Molecular Weight: |
177.16 |
Purity: |
＞98% |
CAS NO#: |
521-31-3 |
Category: |
Trinder's Reagent |
Application: |
Chemiluminescence Immunoassay |
Industry: |
Bioscience |
Luminol, also known as 3-aminophthalazide, is a widely used chemiluminescence reagent for forensic bloodstain detection. In hydrogen peroxide (such as produced by blood breakdown) and alkaline environments, luminol can be activated by catalysts (such as iron ions), emitting blue-green fluorescence, and even trace amounts of blood can be clearly visible in the dark. However, whether the detection efficiency of luminol will be affected when it encounters improper storage or handling, leading to clumping, has become a topic worth exploring in depth.
| Product Name | Luminol/3-aminobenzoyl hydrazine/luminescent ammonia |
| CAS number | 521-31-3 |
| Molecular weight | 177.16 |
| Storage conditions | Room temperature, protected from light and moisture |
| chemical property | Easy to dissolve in alkaline solution, soluble in dilute acid, almost insoluble in water |
| Product Usage | Commonly used in chemiluminescence immunoassay, such as metal cations and blood detection |
| Manufacturer | Hubei Xindesheng Material Technology Co., Ltd |
The detection principle of luminol
The luminescence phenomenon observed during the detection of bloodstains by luminol assay is based on the electron transfer process in chemical reactions. Hemoglobin in blood contains iron element, and when it reacts with oxygen in the air, it releases hydrogen peroxide. Under alkaline conditions, hydrogen peroxide reacts with luminol and catalyst to generate excited 3-aminophthalic acid ions, which release energy in the form of light upon de excitation.
luminol powder
The Causes and Effects of Agglomeration Phenomenon
2.1 Analysis of the causes of agglomeration:
As a chemical reagent, the stability of luminol is influenced by various factors, including temperature, humidity, light, and the sealing of storage containers. When these conditions are not properly controlled, such as prolonged exposure to humid environments or poorly sealed storage containers, luminol powder is prone to absorbing moisture from the air, leading to agglomeration. In addition, vibrations during transportation may also cause particles to squeeze each other, leading to the formation of clumps.
2.2 Impact on detection performance:
(1) Decreased solubility: When preparing a solution, the agglomerated luminol powder may be difficult to dissolve due to the tight binding between particles, resulting in uneven concentration of luminol in the solution and affecting the intensity and uniformity of the luminescence reaction.
(2) Reduced reactivity: During the agglomeration process, the interactions between luminol molecules may change, affecting their activity in chemical reactions, thereby reducing the reaction efficiency with hydrogen peroxide and catalysts, and weakening the luminescence signal.
(3) Decreased detection sensitivity: Due to the above reasons, lumpy luminol may not be able to capture trace amounts of blood when detecting bloodstains, especially those with weaker traces after time and environmental factors, leading to an increased risk of missed detection.
Response measures and solutions
(1) Scientific storage: Ensure that the luminol reagent is stored in a dry, cool, dark, and well ventilated environment, using well sealed containers to reduce contact with moisture and oxygen.
(2) Regular inspection: Regularly check the stored luminol. Once clumping is found, it should be dealt with in a timely manner, and relevant performance indicators should be tested. If any abnormalities occur, it is not suitable for continued use.
Product packaging
Hubei Xindesheng Material Technology Co., Ltd., as an experienced manufacturer of luminol, has strict storage management systems for every batch of luminol powder reagents produced. For the luminol purchased by the customer, sealed and well opaque bottles are used for packaging and transportation to strictly ensure that the product does not clump or deteriorate during transportation. If you are also interested in related products, please click on the official website to learn more details or contact me to discuss more related knowledge!
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