Luminol Reagent 3-Aminophthalhydrazide 521-31-3 Chemiluminescence Immunoassay

Keywords: Luminol, Chemiluminescence

Basic information

Luminol is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst. This catalyst is generally For multivalent metal ions, peroxidases such as iron, horseradish peroxidase, etc., this method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals Toxic analysis and analysis methods based on peroxidase and glucose oxidase.

Luminol Reagent

Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts. The most commonly used catalyst is metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminescence intensity, so that chemiluminescence analysis of certain metal ions can be performed. This reaction can be used to analyze organic compounds containing metal ions to achieve Very high sensitivity. The second is to use the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol to determine the organic compounds that have a quenching effect on the chemiluminescence reaction. The third is the indirect determination of inorganic or organic compounds through coupling reactions.

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Luminol Luminescence Principle

The second is that hydrogen peroxide reacts with sodium hypochlorite to generate oxygen and oxidize luminol to make it glow:
The first is the equation for the reaction of sodium hypochlorite and hydrogen peroxide: NaClO + H2O2 == NaCl + O2 + H2O
Secondly, when luminol reacts with hydroxide, a dianion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. The peroxide is very unstable and immediately decomposes nitrogen (Luminol is not oxidized by organic oxidants such as dimethyl sulfoxide to generate nitrogen, but to generate nitrogen-containing organics), and generates excited 3-aminophthalic acid . In the transition from the excited state to the ground state, the released energy exists in the form of photons with a wavelength in the blue part of visible light.