Blood Collection Tube Additives—Analysis of the phenomenon of serum separation gel oil separation

Serum separation gel, as an important reagent in the biomedical field, plays an indispensable role in the separation and preservation of blood samples. However, in practical use, it is sometimes found that serum separation gel exhibits oil separation phenomenon, which not only affects its separation effect, but may also have adverse effects on experimental results. This article will delve into the reasons for the phenomenon of serum separation gel oil separation.

The basic principle of serum separation gel

We need to understand the basic principle of serum separation gel. Serum separation gel is a viscous fluid with a large number of hydrogen bonds in its structure, which form a network structure through association. Under the action of centrifugal force, the network structure is destroyed and becomes a fluid with low viscosity; After the centrifugal force disappears, the network structure re forms and returns to a high viscosity fluid. This property is called thixotropy and is crucial for serum separation gel

to separate serum and blood cells from blood samples.

Serum separation gel

Analysis of the causes of oil separation phenomenon

1. Quality issues with separation adhesive

The quality of the separation adhesive directly affects its separation effect. If there are problems with the formula or production process of the separation adhesive, it may lead to oil separation during use. For example, certain components in the separation gel may be unstable and prone to changes during storage or transportation, resulting in the formation of oil droplets. In addition, if the initial centrifugal force of the separation adhesive is too large or too small, it may also cause a change in its structure, leading to the phenomenon of oil separation.

2. Improper storage and transportation conditions

Serum separation gel needs to be stored and transported under appropriate temperature and humidity conditions. If the storage temperature is too high or the humidity is too high, it may cause certain components in the separation gel to change, leading to oil separation. In addition, if subjected to severe vibration or collision during transportation, it may also cause changes in the structure of the separation adhesive, leading to the phenomenon of oil separation.

3. Improper operation

When using serum separation gel, it is necessary to follow the prescribed operating steps. If the operation is improper, such as too fast or too slow centrifugation speed, insufficient centrifugation time, etc., it may affect the performance of the separation gel, leading to the occurrence of oil separation phenomenon. For example, excessive centrifugal speed may cause oil droplets in the separation gel to be thrown out; Insufficient centrifugation time may result in incomplete separation of serum and blood cells, leading to the phenomenon of oil separation.

4. Untreated inner wall of test tube

The treatment of the inner wall of the test tube can also affect the separation effect of the serum separation gel. If the inner wall of the test tube is not properly treated, such as without membrane preparation or improper treatment, it may cause friction between the serum separation gel and the inner wall of the test tube during centrifugation, leading to oil separation.

How to avoid oil separation phenomenon

1. Choose high-quality separation adhesive

Choosing a serum separation gel with reliable quality is the key to avoiding oil separation. When purchasing separation adhesive, it is advisable to choose reputable manufacturers and suppliers, and carefully review the product manual and quality certification documents.

2. Strictly control storage and transportation conditions

Serum separation gel needs to be stored and transported under appropriate temperature and humidity conditions. During storage, it should be placed in a cool, dry, and ventilated place, avoiding direct sunlight and high temperature environments. During transportation, measures such as shock and moisture prevention should be taken to ensure that the separation adhesive is not damaged.

3. Standardized operation

When using serum separation gel, the prescribed operating steps should be strictly followed. During the centrifugation process, the centrifugation speed and time should be controlled to ensure complete separation of serum and blood cells. At the same time, attention should also be paid to membrane treatment of the inner wall of the test tube or selecting a treated test tube to reduce friction with the separation gel.

4. Regular inspection and replacement

During use, serum separation gel should be regularly checked and replaced. If oil separation or other abnormal situations are found in the separation adhesive, a new separation adhesive should be replaced in a timely manner to ensure the accuracy and reliability of the experiment.

Product packaging

In summary, the phenomenon of serum separation gel oil separation is a concern. By selecting quality separation glue, strictly controlling storage and transportation conditions, standardizing operations, and regularly inspecting and replacing, we can avoid the occurrence of oil separation and ensure the accuracy and reliability of the experiment.

Hubei Xindesheng Material Technology Co., Ltd., as an established enterprise in the field of blood vessel additives, has always adhered to the principle of market orientation and continuously optimized and innovated its products. In the production process of serum separation gel, every step from the traditional ordinary model to the subsequent anti irradiation version, and now to high-performance products, demonstrates Desheng Company's persistent pursuit of quality and innovation spirit. If you are interested in these cutting-edge products, we sincerely invite you to visit our official website to learn more details!