Analysis of advantages and disadvantages of acridine ester luminescence reaction

The advantages of acridine ester luminescence reaction

1. The luminous system is simple and efficient

One major advantage of acridine ester luminescence reaction is that its reaction system is relatively simple and does not require complex catalysts to participate. In an alkaline environment, hydrogen peroxide reacts with acridine ester molecules to generate unstable ethylene oxide, which then rapidly decomposes into carbon dioxide and electron excited N-methylacridone. When these excited state molecules return to the ground state, they release photons. During this process, no additional energy input is required, the luminescence efficiency is high and the intensity is high, making acridine ester have significant advantages in the field of rapid detection.

acridine ester

2. Fast luminous speed, suitable for fast detection

The rate of chemiluminescence reaction of acridine ester is very fast, usually reaching its maximum luminescence intensity within 0.4 seconds after adding the initiator, with a half-life of only 0.9 seconds. The entire luminescence process is basically completed within 2 seconds. This characteristic makes the acridine ester luminescence reaction perform well in situations where rapid results are needed, such as clinical testing, environmental monitoring, and other fields.

3. Easy to bind with proteins, and the photon yield does not decrease after binding

Acridine ester molecules have good biocompatibility and can easily bind with biomolecules such as proteins. This kind of connection not only does not reduce the photon yield of acridine ester, but also helps to improve its sensitivity and specificity in biological detection. This characteristic makes acridine ester have broad application prospects in fields such as immunoassay and biological probes.

Disadvantages of acridine ester luminescence reaction

1. Poor light stability

Although acridine ester has many advantages, its photostability is relatively poor. Under long-term irradiation, acridine ester molecules are prone to photodegradation, leading to a weakened or even lost luminescence effect. Therefore, when using acridine esters for luminescence reactions, it is necessary to strictly control the lighting conditions to avoid causing light loss.

2. Relatively low reaction selectivity

The selectivity of acridine ester luminescence reaction is relatively low and is easily interfered by other sample components. In complex samples, other components may undergo non-specific reactions with acridine esters, leading to errors in the detection results of the target analyte. To overcome this disadvantage, it is usually necessary to preprocess the sample to eliminate or reduce the influence of interference factors.

3. The storage period of markers is relatively short

The use of acridine ester labeled antibodies has certain limitations in terms of shelf life. Generally speaking, the shelf life of markers is relatively short, usually only about one month. This to some extent limits the use of acridine esters in long-term experiments or large-scale applications. However, with the advancement of technology, researchers are trying to extend the shelf life of markers by adding protective agents and other methods.

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