The Use And Storage Conditions Of Additives For Blood Collection

In clinical testing, the use of blood collection vessels is very extensive. The quality of blood samples and the selection of additives directly affect the accuracy of subsequent detection results. Among them, blood collection additives are directly used in blood collection vessels, which can enhance the stability of blood samples, protect their cell morphology, prolong the preservation of reagents, and improve the accuracy of detection results. However, there are various types and methods of use of additives, and storage conditions also vary.

Additives For Blood Collection

1, Types and effects of blood collection additives

Additives for blood collection mainly include blood anticoagulants, blood coagulants and stabilizers, serum separation gel, etc. The specific blood anticoagulants commonly used are heparin sodium, heparin lithium, EDTA dipotassium, EDTA tripotassium, EDTA disodium, sodium fluoride, etc. According to different projects, the additives used for different anticoagulation principles will also vary.

Blood coagulants mainly accelerate blood coagulation, facilitating the separation of serum and blood cells. They mainly activate platelets and coagulation factors to accelerate blood coagulation; Silicides combine with calcium ions in the blood to form insoluble calcium silicate precipitates, thereby accelerating blood coagulation.

Separation gel: Separation gel is mainly used to separate serum or plasma from red blood cells. The separation gel is mostly a polymer gel with a density between serum and red blood cells. During the centrifugation process, the separation gel effectively separates red blood cells from serum or plasma for subsequent detection.

2, Usage of Additives for Blood Sampling

Select appropriate additives based on the testing items: Different testing items have different requirements for blood samples, so it is necessary to choose appropriate additives based on specific items. For example, blood routine tests typically use EDTA anticoagulants, while coagulation function tests use heparin anticoagulants.

Add according to the specified proportion: The dosage of additives needs to be strictly added in accordance with the instructions or laboratory standard operating procedures. Adding too much or too little may affect the accuracy of the detection results.

Pay attention to the order in which additives are mixed with blood: The order in which certain additives are mixed with blood has an impact on the test results.

Shake well and mix: After adding the additive, the blood collection tube should be gently inverted and shaken several times to fully mix the additive with the blood.

Labeling sample information: After collecting blood samples, the patient's name, gender, age, collection date, and other information should be clearly marked on the blood collection vessel for subsequent testing and identification.

The method for adding additives by the blood vessel manufacturer is as follows:

Cleaning: Before using the machine, it is necessary to clean the tools and conveying pipelines used on the machine. It is recommended to rinse and wipe with deionized water and alcohol, and then air dry before putting it into use.

Machine adjustment and addition: Determine the required additive content based on the production volume of the blood collection vessel, and adjust it to the appropriate numerical range. Continuously stir during the addition process to maintain a uniform suspension. The mixing speed can be adjusted according to the actual situation, and sampling inspection can be conducted to determine whether it is qualified.

Drying: After adding, it needs to be dried with hot air. The temperature is maintained between 40-80 ℃ for 7-120 seconds. If carried out in a drying oven or oven, the temperature should be around 40 degrees Celsius, and the time should be based on complete drying.

Serum separating gel

3, Storage conditions for blood sampling additives

Temperature: Most blood collection additives need to be stored at room temperature (18-25 ℃). However, some additives are sensitive to temperature and need to be stored at low temperatures (2-8 ℃). Therefore, when storing additives, the temperature should be strictly controlled according to the instructions.

Lighting: Additives for blood collection should avoid direct sunlight exposure to avoid changes in the properties of the additives. When storing, try to choose a dark and dark place.

Humidity: Both high and low humidity can affect the stability of blood collection additives. Avoid humid or excessively dry environments when storing.

There are certain requirements and precautions for the use and storage of blood collection additives. Hubei Xindesheng Material Technology Co., Ltd. is an early enterprise that produces a full range of blood collection additives. It has a professional research and development team and can provide technical guidance on use. During the use process, operators should strictly follow the regulations and pay attention to the storage conditions of additives, in order to better ensure the stability of the additives' properties. Only in this way can accurate and reliable detection results be provided for clinical use.