Biological Good Buffer Solutions BICINE CAS 150-25-4 White Crystaline Powder 99%

Bicine is an amino acid derivative, weakly acidic, pH buffer range is 7.6-9.0, soluble in water and commonly used organic solvents such as DMF, DMSO, DMAc, etc.When powdered bicine is formulated into a solution, sodium hydroxide is usually added to adjust the pH value to meet the pH environmental requirements required for the experiment or detection.If the experimental environment needs to exclude the interference of sodium ions, potassium hydroxide can be used instead of sodium hydroxide, and then the pH is measured by a pH meter to adjust the mixing ratio of Bicine solution and alkali solution.

Basic Information

Description

Bicine is one of the commonly used biological buffers. It is an amphoteric amino acid buffer and has activity in the pH range of 7.6-9.0 (pKa of 8.3 at 25 ° C). Recommended buffer for low temperature biochemical work. Bicaine is used to prepare a stable serum guanosidase substrate solution. A method for protein separation using dihydropyridine in thin layer ion exchange chromatography has been published. Dihydropyridine has been used for peptide and protein crystallization.

Applications of N, N-dihydroxyethylglycine (Bicine)

1. The molecular structure of Bicine is similar to glycine. The molecule has a substituted amino group, a carboxyl group and 2 hydroxyl groups as coordination groups, so it has the properties of both amino acid and aminoethanol, and can form a metal with a single anion (Bic-) The complex is often used as a buffer solution for capillary electrophoresis.

2. According to the description of patent CN104245089A, Bicine is suitable as an effective inhibitor of oxidative degradation of amines, and can effectively inhibit the oxidative degradation of amines used or amine-containing absorbents used.

3.Used to prepare a stable substrate solution for serum guanine enzyme determination. It is also a very widely used Buffer, used in enzyme reaction buffer, electrophoresis buffer, working concentration from 3-100mM.

4.Bicine is an amino acid analog that is easily soluble in water. It is an environmentally friendly substance. The molecule contains two -OH and one -COOH. It has good chelating properties and can chelate heavy metal ions such as Cu, Cd, and Pb. , but can not chelate calcium and magnesium ions. Therefore, Bicine is often used as a chelating agent to repair heavy metal contaminated soil. Bicine has a good elution capacity for heavy metals under neutral and acidic conditions, and can efficiently remove Cu, Cd, and Pb ions in contaminated soil. Bicine is the eluent of the active component, which can not only rinse and repair heavy metal contaminated soil, but also avoid the loss of plant nutrient elements calcium and magnesium in the soil. It is safe and environmentally friendly, and will not cause secondary pollution to the environment.

Product packaging

Advantage

The purity (> 99%) is water-soluble and the process is stable.

1. Bicine is often used as a buffer solution for capillary electrophoresis

Bicine is a zwitterionic buffer, belonging to Good’s buffer, with a pH buffer range of 7.6-9.0. The molecular structure of Bicine is similar to that of glycine. The molecule has a substituted amino group, a carboxyl group and two hydroxyl groups as coordinating groups, so it has the properties of amino acid and aminoethanol at the same time. It can form metal complexes with a single anion and is often used. Used as a buffer solution for capillary electrophoresis.

2. Bicine is an effective inhibitor for the oxidative degradation of amines

According to the description of patent CN104245089A, Bicine is an effective inhibitor for the oxidative degradation of amines, and can effectively inhibit the oxidative degradation of the amine used or the amine-containing absorbent used.

3. Bicine is used as a chelating agent for soil remediation

Bicine is an amino acid analogue that is easily soluble in water. It is an environmentally friendly substance. It contains two -OH and one -COOH in the molecule. It has good chelating properties and can chelate heavy metal ions such as Cu, Cd, and Pb. Can not chelate calcium and magnesium ions. Therefore, Bicine is often used as a chelating agent to repair heavy metal contaminated soil. Bicine has good elution ability for heavy metals under neutral and acidic conditions, and can efficiently remove Cu, Cd, Pb ions in contaminated soil. Bicine is the eluent of active components, which can not only wash and repair heavy metals It pollutes the soil, and can avoid the loss of plant nutrients calcium and magnesium in the soil, is safe and environmentally friendly, and will not cause secondary pollution to the environment.

Bicine buffer is also a widely used in vitro diagnostic reagent raw material, suitable for biochemistry and medicine. In the field of in vitro diagnostics, it is an important raw material for antibody detection reagents and nucleic acid detection reagents.