CAS 1135-40-6 CAPS Biological Buffer 3-Cyclohexylaminopropanesulfonic Acid

Application of biological buffer CAPS

CAPS, also known as 3-cyclohexylaminopropanesulfonic acid, is a biological buffering agent. It is white crystal or powder in appearance. CAPS can be dissolved in water. The pH buffer range is 8.9-10.3. The PKA value is 9.6 and the molecular weight is 243.28 at 25℃. The CAS number is 1135-40-6. CAPS buffer has a wide range of applications, including DNA/RNA extraction kits, PCR diagnostic kits, biochemical diagnostic kits, manufacturing welding materials, air conditioning equipment, manufacturing metal lithium, new coatings, new industrial materials, etc.

Brief introduction

CAPS white crystalline powder

Buffer capacity of 3-(cyclohexylamine)-1-propanesulfonic acid buffer solution:

Adding a small amount of strong acid or alkali to the buffer solution will not change the pH value of the solution much, but if the amount of acid and alkali is large, the buffer solution will lose its buffering effect. This shows that its buffer capacity is limited.
The buffer capacity of the buffer solution is related to the concentration of the components that make up the buffer solution. The buffer solution composed of 0.1mol·L-1HAc and 0.1mol·L-1NaAc has a greater buffering capacity than the buffer solutions of 0.01mol·L-1HAc and 0.01mol·L-1NaAc. This point can be confirmed by calculation. But the concentration of the components of the buffer solution should not be too high, otherwise, the effect between ions cannot be ignored.

CAPS powder

When the ratio of the two components of the buffer solution is not 1:1, the buffering effect is reduced and the buffering capacity is reduced. When c (salt)/c (acid) is 1:1, the △pH is the smallest and the buffering capacity is large. Whether for acid or alkali, it has a greater buffering effect. The pH value of the buffer solution can be calculated by the following formula:
At this time, the buffer capacity is large. The farther the ratio of the buffering components is from 1:1, the smaller the buffering capacity, and it may not even play a buffering role. For any buffer system, there is an effective buffer range, which is roughly within one pH unit on each side of pKaφ (or pKbφ).

Weak acid and its salt (weak acid and its conjugate base) system pH=pKaφ±1
Weak base and its salt (weak base and its conjugate acid) system pOH=pKbφ±1

For example, the pKaφ of HAc is 4.76, so HAc and NaAc are suitable for preparing a buffer solution with a pH of 3.76 to 5.76, which has a greater buffering effect in this range. The buffering capacity is the largest when preparing a buffer solution with pH=4.76, at this time (c(HAc)/c(NaAc)=1. 3-(cyclohexylamine)-1 propanesulfonic acid has a wide range of uses, 3-cyclohexylaminopropanesulfone Acid (CAPS) is relatively stable in nature, with a pKa value of 10.4 at 25°C and a pH buffer range of 9.7-11.1. It is widely used in biochemical analysis and in vitro diagnostic industries.

Product packaging

Desheng Bio has been engaged in the research and development and production of biological buffering agent projects for many years and has very rich experience. CAPS is one of the core products, and it is well received in the field of biochemical diagnostic reagents and the new coating supplies market. Desheng has established a company for decades. The company's products are sold globally. At present, it has been sold to more than 100 countries around the world. The products are of good quality and more favorable prices. If you are interested, you can call for consultation. Desheng Bio welcomes your calls.