Strongly Chemiluminescent Acridinium Esters Invitro Immunoassays Using

Luminol, also known as luminol. A blood that cannot be observed with the naked eye when detected at a crime scene, and can show a very small amount of blood (occult blood reaction). Seeing this, I think this product is so powerful, it is a magic weapon for solving crimes! However, it is not only used in solving crimes!

Let's look down, its chemical name is 5-amino-phthalic acid hydrazide. It is a blue crystal or light yellow powder at room temperature, and it is a relatively stable synthetic organic compound. The chemical formula is C8H7N3O2. At the same time, luminol is a strong acid, which has a certain irritating effect on the eyes, skin and respiratory tract. From the picture below we can see that it is still very beautiful with a blue shimmer, but often the more beautiful things are, the more dangerous it is. Therefore, we should not directly touch it with our hands or the epidermis, so as not to be hurt by it.

Luminol powder

It is generally used for chemiluminescence analysis, such as: metal cations, blood and glucocorticoids. Luminescence test: Emmax440Chemicalbooknm (chemiluminescence; 60mMK2S2O8, 100mMK2CO3, pH11.5; after adding H2O2) chemiluminescence reagents and indicators, often used in chemiluminescence analysis, such as metal cations, blood immunity and so on. Their greatest advantage is their high sensitivity.

In addition, because the hemoglobin in our blood contains iron, and iron can catalyze the decomposition of hydrogen peroxide, the hydrogen peroxide becomes water and monooxygen, and the monooxygen oxidizes luminol to make it glow. Therefore, luminol is widely used in criminal investigation, biological engineering, chemical tracing and other fields. In forensic medicine, luminol reaction can identify blood stains that have been scrubbed long ago. Biologically, luminol is used to detect the presence of copper, iron and cyanide in cells. This is its power. It is easily soluble in lye, soluble in dilute acid, almost insoluble in water, and hardly soluble in alcohol. Neutral or slightly acidic solutions show strong bright blue fluorescence when exposed to ultraviolet light. The optimal fluorescence wavelength is 425nm (check the chemiluminescence rate in 60mMK2S2O8, 100mK2CO3, PH11.5 solution).

But it also has disadvantages:

1. Luminol fluoresces in the presence of iron, iron-containing alloys, horseradish or certain bleaching agents. Therefore, if the crime scene is thoroughly treated with bleach, the fluorescence from luminol will strongly mask any blood stains.

2. Luminol can detect a small amount of animal blood and urine, so if the room to be tested contains urine or animal blood, the test results will be biased.

3. Luminol reacts with excrement, and the light emitted is the same as that emitted by blood.

4. Luminol may interfere with other tests, but luminol does not interfere with DNA extraction.

5. The use of luminol needs to be in a dark environment, otherwise the fluorescence is difficult to identify.

6. Luminol has a limited amount of time to glow, so you must hurry up to take photos and record.

Product packaging

Desheng Technology will continue to develop and research advanced biochemical reagents around the needs of the medical laboratory field to meet the needs of the global medical laboratory technology development and strive for the cause of human health. Everyone is welcome to consult.