Strongly 98% Acridinium Ester chemiluminescence Acridinium Ester Trigger Solution

In the field of chemiluminescence analysis, the advantages of the chemiluminescence reagent acridine ester NSP-DMAE-NHS without the need for catalysts are demonstrated in terms of simplicity of the luminescence system, reduced background luminescence, and reduced interference. These advantages also make it widely used in various fields of chemiluminescence analysis, such as biochemical research, environmental monitoring, clinical testing, etc.

Simplicity of luminous system

1. The catalytic free reaction mechanism basis: The core of the acridine ester NSP-DMAE-NHS luminescent system lies in its reaction process under alkaline conditions. In alkaline environments, acridine ester molecules can directly react with hydrogen peroxide. Hydrogen peroxide attacks acridine ester molecules, promoting their formation of ethylene oxide. This process does not require additional catalyst intervention and is based on the chemical activity and structural characteristics of acridine ester molecules themselves. Specific functional groups and chemical bonds in its molecular structure can undergo redox reactions with hydrogen peroxide under alkaline conditions, thereby initiating luminescent reactions. This reaction mechanism based on its own structural characteristics makes the composition of the luminescent system relatively simple, reduces the dependence on external catalysts, and lowers the complexity of the reaction system.

acridine ester NSP-DMAE-NHS powder

2. Compared with traditional luminescence systems, many traditional chemiluminescence reactions often require catalysts to accelerate the reaction process or change the reaction pathway to achieve detectable luminescence signals. For example, certain enzyme catalyzed chemiluminescence reactions require specific enzymes as catalysts, which not only increases the variety and cost of reagents, but also requires strict control over enzyme activity, stability, and other factors. The NSP-DMAE-NHS catalyst free luminescent system avoids these problems. In experimental operations and practical applications, there is no need for additional steps to add, store, and handle catalysts, simplifying the experimental process.

Reduced background illumination

1. Catalyst free reduction of non-specific reactions: Due to the lack of catalyst, the acridine ester NSP-DMAE-NHS luminescence system reduces background luminescence. In chemiluminescence analysis, background luminescence mainly comes from weak luminescence signals generated by non-specific reactions, which can interfere with the detection accuracy of the target analyte. The presence of catalysts may sometimes trigger some side reactions, and the NSP-DMAE-NHS luminescent system has a relatively single and clear reaction pathway without catalysts, reducing the probability of non-specific reactions.

2. The significance of improving detection sensitivity: The reduction of background luminescence directly enhances the sensitivity of detection. In chemiluminescence detection, the detection sensitivity depends on the ratio of the target signal to the background signal. When the background luminescence decreases, even if the luminescence signal generated by the target analyte is relatively weak, it can be more easily distinguished from the background, which is particularly important for the detection of low concentration analytes.

Reduce interference

Acridine ester NSP-DMAE-NHS does not require a catalyst to avoid catalyst related interference factors. The catalyst itself may interact with certain components in the sample, changing the chemical composition or physical state of the sample, thereby affecting the accuracy of the luminescence reaction. For example, some metal ion catalysts may undergo complexation reactions with proteins, nucleic acids, etc. in biological samples, which not only affect the activity of the catalyst, but may also generate additional luminescence or absorption signals, interfering with the detection of target analytes.

Product packaging

Hubei Xindesheng, as a research and development manufacturer of acridine ester NSP-DMAE-NHS raw materials, provides high-quality and diverse acridine ester series products. The company relies on a professional research team and technology to continuously explore the chemical properties of acridine esters and optimize the synthesis process. The products produced have high purity and sensitive chemiluminescence properties. Whether used for clinical diagnostic reagent development or biological research experiments, Xindesheng's acridine ester series products can meet the needs. If you are interested, please feel free to click for consultation at any time!