CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid TAPS Buffer

Taps, n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, is a member of the good's buffer family. It is a kind of amphoteric buffer widely used in biochemistry and molecular biology. It is often used as a buffer for the separation of dyes in planar chromatography and for the purification of enzymes in ion exchange chromatography.

Chromatography is a laboratory technique, usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Since the retention of ionizable compounds is particularly sensitive to the pH of the mobile phase, a buffer should be added to the system to control this variable.

TAPS powder

Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers including taps have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.

2, One of the most suitable biological buffers for cell culture

As mentioned above, taps is a member of the good's family of buffers. One of the most important features of good's buffers is that they are non-toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH value under control. Due to their special functions, many good's Buffers / biological buffers are considered ideal for cell isolation, cell culture, enzyme analysis and many other biochemical applications.

Desheng's researchers found that protonated sulfamate compounds directly inhibit the activity of connexin channels. In good's pH buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] - 1), pH dependent connexin channel activity demonstrated this. -Propane sulfonic acid), which share a common sulfamate moiety and have no pH dependent channel activity in pH buffer without sulfamate moiety. Therefore, taps buffer is more used for flagellate algae experiment in cell culture medium.

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