The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic detection and biochemical tests. In the colorimetric determination of hydrogen peroxide activity, it has several advantages over conventional chromogenic reagents.
The new Trinder's reagent is stable enough to be used in both solution and test line detection system. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent formed very stable purple or blue dyes in the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolylsulfonylhydrazone (MBTH). The molar absorptivity of the dye coupled with MBTH is 1.5-2 times higher than that of the dye coupled with 4-AA; however, 4-AA solution is more stable than MBTH solution.
The substrate is oxidized by its oxidase to produce hydrogen peroxide, and the concentration of hydrogen peroxide corresponds to the concentration of the substrate.
Glucose, alcohol, acyl coenzyme A and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA.
Trinder's reaction, also known as "coupling end-point colorimetry", or enzyme method, is mainly used for the detection of uric acid, creatinine, blood glucose, cholesterol, triglyceride and so on in blood test. Trinder's reaction is the basis of enzymatic determination of glucose, cholesterol, triglyceride and uric acid.
In Trinder's reaction, the chromogen or chromogenic agent is Trinder's reagent, also known as chromogen substrate or hydrogen donor. Phenols include phenol, 4-chlorophenol or sodium 3,5-dichloro-2-hydroxybenzenesulfonate; aniline includes N, n-dialkylaniline, N, n-dialkyl-m-toluidine, etc.
Desheng company has made its own way in the industry in the past 15 years. In the small branch of in vitro diagnostic reagents, Desheng also plays its own R & D ability, taking the existing chromogen substrates (the New Trinder's reagents) such as toos, tops, ADOS, ADPS, Alps, Daos, hdaos, MADB, Maos, todb and other reagent products as a separate R & D field, and constantly developing them Innovative research has been in production capacity since 2012. After years of exploration, the in vitro diagnostic reagent products have been continuously improved. Compared with the previous new reagents, it has more advantages: such as high water solubility, UV absorption of color reaction products > 540nm; color reaction requires a wider pH range, which can ensure the activity of a variety of enzymes; color reaction has higher sensitivity, It can be used for the determination of very small amount of analytes.
